Active Recombinant Human EGFR Protein, His-Avi-tagged, Biotinylated
Cat.No. : | EGFR-288H |
Product Overview : | Biotinylated Human EGFRvIII is expressed from HEK293 cells. It contains AA Leu 25 - Ser 645 (30-297: Val Cys Gln...Cys Pro Arg → Gly) (Accession # NP_005219.2). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. |
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Source : | HEK293 |
Species : | Human |
Tag : | His-Avi |
Predicted N Terminal : | Leu 25 |
Form : | Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
Bio-activity : | Immobilized CetuxiMabat 2 ug/mL (100 uL/well) can bind Biotinylated Human EGFRvIII with a linear range of 0.4-25 ug/mL. |
Molecular Mass : | The protein has a calculated MW of 41.3 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing condition, and 60-70 kDa under non-reducing condition (SDS-PAGE). |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Characteristic : | Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
Products Types
◆ Lysates | ||
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EGFR-2733HCL | Recombinant Human EGFR cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionIt is associated with biological processes in human embryonic development and tissue regeneration.
EGFR inhibits adverse drug reactions such as rash, inflammation, and diarrhea during drug treatment.
The role of EGFR in tumor treatment is to act as a molecular marker and can be used as a prognostic indicator and monitor tumor progression; at the same time, EGFR mutations can also be targeted for targeted therapy.
EGFR gene mutation detection generally uses PCR, fluorescence in situ hybridization, and direct sequencing.
The combination of EGFR inhibitors with chemotherapy drugs, radiotherapy, immunotherapy, etc. can increase the efficacy of treatment and reduce the occurrence of adverse reactions.
EGFR mutations can have a significant impact on the efficacy of targeted therapies, and patients with mutations have higher response rates.
Customer Reviews (3)
Write a reviewThe powerful biological activity of EGFR combined with its high purity makes the experimental results highly reproducible, which is of great significance in the field of scientific research.
Due to the short half-life and high clearance characteristics of EGFR, they can better adapt to the needs of clinical treatment and provide more treatment options for patients.
The production process of EGFR uses advanced biotechnology, which makes the production process more efficient and environmentally friendly, and reduces the consumption of human and material resources.
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