Recombinant Human FOXA1 293 Cell Lysate

• Cat.No.: FOXA1-6164HCL

Specification

• Description: Antigen standard for forkhead box A1 (FOXA1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: FOXA1 forkhead box A1 [ Homo sapiens ]
• Official Symbol: FOXA1
• Synonyms: FOXA1; forkhead box A1; hepatocyte nuclear factor 3, alpha , HNF3A; hepatocyte nuclear factor 3-alpha; HNF-3A; TCF-3A; HNF-3-alpha; forkhead box protein A1; transcription factor 3A; hepatocyte nuclear factor 3, alpha; HNF3A; TCF3A; MGC33105
• Gene ID: 3169
• mRNA Refseq: NM_004496
• Protein Refseq: NP_004487
• MIM: 602294
• UniProt ID: P55317
• Chromosome Location: 14q12-q13
• Pathway: Direct p53 effectors, organism-specific biosystem; FOXA transcription factor networks, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem
• Function: DNA binding; DNA binding, bending; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; double-stranded DNA binding; protein domain specific binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription factor binding; transcription regulatory region DNA binding

Reviews

02/03/2023

The company provides a dedicated team of experts who are readily available to assist researchers throughout their experimental journey.

12/15/2022

Whether it be troubleshooting issues, offering experimental guidance, or addressing any concerns, the manufacturer's technical support ensures that researchers have a reliable and knowledgeable resource to rely on.

06/11/2017

In addition to its scientific merits, the manufacturer of FOXA1 protein stands out for its outstanding technical support.

Q&As

How does FOXA1 protein contribute to the development of liver cancer?

FOXA1 has been implicated in the development and progression of hepatocellular carcinoma, with its expression levels serving as a potential prognostic marker.

Is FOXA1 protein involved in the regulation of estrogen receptor signaling?

Yes, FOXA1 is a key co-regulator of estrogen receptor (ER) signaling, influencing the expression of ER-responsive genes and impacting the response to anti-estrogen therapies.

Can FOXA1 protein expression be used as a predictive biomarker in lung cancer?

FOXA1 expression has been associated with specific subtypes of lung cancer and may hold potential as a predictive biomarker for treatment response.

Are there any clinical trials investigating the role of FOXA1 protein in cancer treatment?

Yes, there are clinical trials focused on understanding the role of FOXA1 in cancer and exploring its potential as a therapeutic target in various cancer types.

How does FOXA1 protein influence the response to anti-androgen therapy in prostate cancer?

High FOXA1 expression has been linked to resistance to anti-androgen therapy in prostate cancer, highlighting its clinical relevance in treatment decision-making.