Recombinant Human GPC3 Protein (25-559aa), C-His-tagged

• Cat.No.: GPC3-22H
• Product Overview: Recombinant human GPC3 (25-559aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
• Source: Baculovirus
• Species: Human
• Tag: C-His
• Form: Liquid
• Molecular Mass: 61.8 kDa (544aa)
• Protein Length: 25-559
• AA Sequence: ADPQPPPPPPDATCHQVRSFFQRLQ PGLKWVPETPVPGSDLQVCLPKGPT CCSRKMEEKYQLTARLNMEQLLQSA SMELKFLIIQNAAVFQEAFEIVVRH AKNYTNAMFKNNYPSLTPQAFEFVG EFFTDVSLYILGSDINVDDMVNELF DSLFPVIYTQLMNPGLPDSALDINE CLRGARRDLKVFGNFPKLIMTQVSK SLQVTRIFLQALNLGIEVINTTDHL KFSKDCGRMLTRMWYCSYCQGLMMV KPCGGYCNVVMQGCMAGVVEIDKYW REYILSLEELVNGMYRIYDMENVLL GLFSTIHDSIQYVQKNAGKLTTTIG KLCAHSQQRQYRSAYYPEDLFIDKK VLKVAHVEHEETLSSRRRELIQKLK SFISFYSALPGYICSHSPVAENDTL CWNGQELVERYSQKAARNGMKNQFN LHELKMKGPEPVVSQIIDKLKHINQ LLRTMSMPKGRVLDKNLDEEGFESG DCGDDEDECIGGSGDGMIKVKNQLR FLAELAYDLDVDDAPGNSQQATPKD NEISTFHNLGNVHHHHHHH
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: GPC3 glypican 3 [ Homo sapiens (human) ]
• Official Symbol: GPC3
• Synonyms: GPC3; glypican 3; glypican-3; glypican proteoglycan 3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
• Gene ID: 2719
• mRNA Refseq: NM_004484
• Protein Refseq: NP_004475
• MIM: 300037
• UniProt ID: P51654