Recombinant Human IL12 Protein, His-Avi-tagged

• Cat.No.: IL12-433H
• Product Overview: Recombinant Human IL12 Protein is expressed from mammalian with a His Tag and Avi Tag at the C-terminal. It contains Ile23-Ser328 (IL12B/P40)/Arg23-Ser219(P35/IL12A).

Specification

• Description: Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Protein length: Ile23-Ser328 (IL12B/P40)/Arg23-Ser219(P35/IL12A)
• Endotoxin: Less than 0.1 EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE. > 95% as determined by HPLC.
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL12B interleukin 12B [ Homo sapiens (human) ]
• Official Symbol: IL12
• Synonyms: CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• Gene ID: 3593
• mRNA Refseq: NM_002187.3
• Protein Refseq: NP_002178.2
• MIM: 161561
• UniProt ID: P29460

Reviews

01/07/2022

Effective in Th1 cell differentiation.

04/09/2020

High specificity in ELISA.

08/02/2018

Stable under storage.

Q&As

How does fluorescence microscopy track IL12 internalization and intracellular trafficking?

Fluorescence microscopy tracks IL12 internalization and trafficking.

What techniques dissect IL12's signaling cascades and crosstalk with other cytokines?

Phosphoproteomics and flow cytometry dissect IL12's signaling and crosstalk.

What strategies assess IL12's polarization of T cell subsets and cytokine profiles?

Flow cytometry and ELISA assess IL12's T cell subset polarization and cytokine profiles.

How are reporter gene assays tailored to study IL12's promoter activity and response elements?

Reporter gene assays analyze IL12's promoter activity and response elements.

What insights into IL12's cellular distribution and dynamics do super-resolution imaging methods provide?

Super-resolution imaging details IL12's cellular distribution and dynamics.

What are the benefits of using 3D cell culture models to investigate IL12's effects on tissue microenvironments?

3D cell culture models reveal IL12's effects on tissue microenvironments.

How do protein-protein interaction assays decipher IL12's binding partners in cellular context?

Co-localization studies and co-IP assays identify IL12's binding partners.

What methods elucidate IL12's receptor complex formation and stoichiometry?

Techniques like co-immunoprecipitation reveal IL12 receptor complex assembly.

Can you elaborate on the utility of ChIP-seq in understanding IL12-regulated gene expression networks?

ChIP-seq helps map IL12-induced gene expression patterns and regulatory regions.

Could you explain how transgenic models help dissect IL12's roles in immune response modulation?

Transgenic models elucidate IL12's roles in immune response regulation.