Recombinant Human IL12RB1

• Cat.No.: IL12RB1-8326H
• Product Overview: Recombinant Human IL12RB1 was expressed in NSO Cells.

Specification

• Description: Interleukin 12 receptor, beta 1 (IL12RB1), also known as CD212, is a type I transmembrane protein that belongs to the gp130/G-CSF receptor family. It is a common subunit of both the IL-12 and IL-23 receptor complexes which play roles in T cell mediated inflammatory reactions. IL12RB1 binds IL-12 with low affinity and is involved in IL-12 transduction. In association with IL23R, it forms the IL-23 receptor complex which functions in IL-23 signal transduction probably through activation of the Jak-STAT signaling cascade. IL12RB1 is expressed on activated T cells, B cells, NK cells, macrophages, and microglia. Defects in IL12RB1 are generally associated with selective susceptibility to weakly pathogenic mycobacteria and Salmonella species. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Source: Mammalian cells
• Species: Human
• Form: This recombinant protein was 0.2 μm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Molecular Mass: The predicted molecular weight of Recombinant Human IL-12 Rβ1 is Mr 84.5 kDa (monomer). However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 110 kDa.
• AA Sequence: crtsecc fqdppypdad sgsasgprdl rcyrissdry ecswqyegpt agvshflrcc lssgrccyfa agsatrlqfs dqagvsvlyt vtlwveswar nqtekspevt lqlynsvkye pplgdikvsk lagqlrmewe tpdnqvgaev qfrhrtpssp wklgdcgpqd ddtesclcpl emnvaqefql rrrqlgsqgs swskwsspvc vppenppqpq vrfsveqlgq dgrrrltlke qptqlelpeg cqglapgtev tyrlqlhmls cpckakatrt lhlgkmpyls gaaynvavis snqfgpglnq twhipadtht epvalnisvg tngttmywpa raqsmtycie wqpvgqdggl atcsltapqd pdpagmatys wsresgamgq ekcyyitifa sahpekltlw stvlstyhfg gnasaagtph hvsvknhsld svsvdwapsl lstcpgvlke yvvrcrdeds kqvsehpvqp tetqvtlsgl ragvaytvqv radtawlrgv wsqpqrfsie iegrmdpksc dkthtcppcp apellggpsv flfppkpkdt lmisrtpevt cvvvdvshed pevkfnwyvd gvevhnaktk preeqynsty rvvsvltvlh qdwlngkeyk ckvsnkalpa piektiskak gqprepqvyt lppsrdeltk nqvsltclvk gfypsdiave wesngqpenn ykttppvlds dgsfflyskl tvdksrwqqg nvfscsvmhe alhnhytqks lslspgkhhh hhh
• Endotoxin: <1.0 eu/μg as determined by the lal>
• Purity: >95% by SDS-PAGE and analyzed by silver stain.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: IL12RB1 interleukin 12 receptor, beta 1 [ Homo sapiens ]
• Official Symbol: IL12RB1
• Synonyms: IL12RB1; interleukin 12 receptor, beta 1; IL12RB; interleukin-12 receptor subunit beta-1; CD212; IL-12RB1; IL-12R-beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; interleukin-12 receptor beta-1 chain; IL-12R-BETA1; MGC34454
• Gene ID: 3594
• mRNA Refseq: NM_005535
• Protein Refseq: NP_005526
• MIM: 601604
• UniProt ID: P42701
• Chromosome Location: 19p13.1
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; IL12-mediated signaling events, organism-specific biosystem; IL23-mediated signaling events, organism-specific biosystem; IL27-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine receptor activity; contributes_to interleukin-12 receptor activity; contributes_to interleukin-12 receptor binding; contributes_to interleukin-23 binding; contributes_to interleukin-23 receptor activity; receptor activity

Reviews

04/06/2022

Reliable in co-immunoprecipitation.

10/18/2021

Effective in asthma models.

12/21/2017

great in Western blot analysis.

Q&As

Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization?

Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation?

RNA-seq profiles gene expression changes upon IL12RB1 activation.

What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation?

ChIP-seq investigates IL12RB1's impact on gene regulation networks.

What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A?

Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments?

Knockdown experiments reveal IL12RB1's contribution to signal transduction.

What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways?

siRNA screening identifies pathways influenced by IL12RB1 expression.

How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions?

Co-crystallization offers structural insights into IL12RB1 interactions.

How does flow cytometry provide insights into IL12RB1 expression levels on different cell types?

Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses?

Functional assays validate IL12RB1's role in immune responses.