Recombinant Human IL12RB1 Protein (Pro37-Glu234), His tagged

• Cat.No.: IL12RB1-3372H
• Product Overview: Recombinant human IL12RB1 (Pro37-Glu234) protein fused with His tag was expressed in E. coli.

Specification

• Description: Interleukin12 receptor subunit beta 1 (IL12RB1) is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. IL12RB1 can spontaneously form homodimers and -oligomers, which are able to bind IL12 with only low affinity. IL12 high affinity receptor complex is composed of two subunits designated IL12RB1 and IL12RB2. While IL12RB1 interacts with the IL-12p40 subunit, IL-12p35 is mainly connecting with IL12RB2. This receptor chain is also responsible for transmitting the IL12 signal into the cell. IL12RB1, to the contrary, is also part of the IL23R, where it interacts with the p40 subunit of IL23. IL12RB1 is expressed in activated T cells, NK cells and B cells.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 24.21 kDa
• Protein Length: Pro37-Glu234
• Purity: >90 % as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS pH 7.5 or lyophilized from PBS pH 7.5.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: IL12RB1 interleukin 12 receptor, beta 1 [ Homo sapiens (human) ]
• Official Symbol: IL12RB1
• Synonyms: IL12RB1; interleukin 12 receptor, beta 1; IL12RB; interleukin-12 receptor subunit beta-1; CD212; IL-12RB1; IL-12R-beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; interleukin-12 receptor beta-1 chain; IL-12R-BETA1; MGC34454
• Gene ID: 3594
• mRNA Refseq: NM_005535
• Protein Refseq: NP_005526
• MIM: 601604
• UniProt ID: P42701

Reviews

04/06/2022

Reliable in co-immunoprecipitation.

10/18/2021

Effective in asthma models.

12/21/2017

great in Western blot analysis.

Q&As

Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization?

Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation?

RNA-seq profiles gene expression changes upon IL12RB1 activation.

What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation?

ChIP-seq investigates IL12RB1's impact on gene regulation networks.

What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A?

Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments?

Knockdown experiments reveal IL12RB1's contribution to signal transduction.

What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways?

siRNA screening identifies pathways influenced by IL12RB1 expression.

How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions?

Co-crystallization offers structural insights into IL12RB1 interactions.

How does flow cytometry provide insights into IL12RB1 expression levels on different cell types?

Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses?

Functional assays validate IL12RB1's role in immune responses.