Recombinant Human IL1B Protein, GST-tagged

• Cat.No.: IL1B-52H
• Product Overview: Recombinant Human IL1B Protein, fused to GST-tag, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Supplied as a 0.2 μm filtered solution in PBS, 15%glycerol, pH8.0.
• Molecular Mass: ~57.3 kDa
• AA Sequence: MSPILGYWKIKGLVQPTRLLLEYLE EKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYI ADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSK LPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPK LVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRG SMAEVPELASEMMAYYSGNEDDLFF EADGPKQMKCSFQDLDLCPLDGGIQ LRISDHHYSKGFRQAASVVVAMDKL RKMLVPCPQTFQENDLSTFFPFIFE EEPIFFDTWDNEAYVHDAPVRSLNC TLRDSQQKSLVMSGPYELKALHLQG QDMEQQVVFSMSFVQGEESNDKIPV ALGLKEKNLYLSCVLKDDKPTLQLE SVDPKNYPKKKMEKRFVFNKIEINN KLEFESAQFPNWYISTSQAENMPVF LGGTKGGQDITDFTMQFVSSAAAS
• Purity: >90%
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.17 mg/ml

Gene Information

• Gene Name: IL1B interleukin 1 beta [ Homo sapiens (human) ]
• Official Symbol: IL1B
• Synonyms: IL-1; IL1F2; IL1beta; IL1-BETA
• Gene ID: 3553
• mRNA Refseq: NM_000576
• Protein Refseq: NP_000567
• MIM: 147720
• UniProt ID: P01584