Recombinant Human ILF3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ILF3-3010H
• Product Overview: ILF3 MS Standard C13 and N15-labeled recombinant protein (NP_036350) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 95.2 kDa
• AA Sequence: MRIFVNDDRHVMAKHSSVYPTQEEL EAVQNMVSHTERALKAVSDWIDEQE KGSSEQAESDNMDVPPEDDSKEGAG EQKTEHMTRTLRGVMRVGLVAKGLL LKGDLDLELVLLCKEKPTTALLDKV ADNLAIQLAAVTEDKYEILQSVDDA AIVIKNTKEPPLSLTIHLTSPVVRE EMEKVLAGETLSVNDPPDVLDRQKC LAALASLRHAKWFQARANGLKSCVI VIRVLRDLCTRVPTWGPLRGWPLEL LCEKSIGTANRPMGAGEALRRVLEC LASGIVMPDGSGIYDPCEKEATDAI GHLDRQQREDITQSAQHALRLAAFG QLHKVLGMDPLPSKMPKKPKNENPV DYTVQIPPSTTYAITPMKRPMEEDG EEKSPSKKKKKIQKKEEKAEPPQAM NALMRLNQLKPGLQYKLVSQTGPVH APIFTMSVEVDGNSFEASGPSKKTA KLHVAVKVLQDMGLPTGAEGRDSSK GEDSAEETEAKPAVVAPAPVVEAVS TPSAAFPSDATAEQGPILTKHGKNP VMELNEKRRGLKYELISETGGSHDK RFVMEVEVDGQKFQGAGSNKKVAKA YAALAALEKLFPDTPLALDANKKKR APVPVRGGPKFAAKPHNPGFGMGGP MHNEVPPPPNLRGRGRGGSIRGRGR GRGFGGANHGGYMNAGAGYGSYGYG GNSATAGYSQFYSNGGHSGNASGGG GGGGGGSSGYGSYYQGDNYNSPVPP KHAGKKQPHGGQQKPSYGSGYQSHQ GQQQSYNQSPYSNYGPPQGKQKGYN HGQGSYSYSNSYNSPGGGGGSDYNY ESKFNYSGSGGRSGGNSYGSGGASY NPGSHGGYGGGSGGGSSYQGKQGGY SQSNYNSPGSGQNYSGPPSSYQSSQ GGYGRNADHSMNYQYRTRTRPLEQK LISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ILF3 interleukin enhancer binding factor 3 [ Homo sapiens (human) ]
• Official Symbol: ILF3
• Synonyms: ILF3; interleukin enhancer binding factor 3, 90kDa; interleukin enhancer binding factor 3, 90kD; interleukin enhancer-binding factor 3; DRBP76; M phase phosphoprotein 4; MPHOSPH4; MPP4; NF90; NFAR 1; M-phase phosphoprotein 4; translational control protein 80; dsRNA binding protein NFAR-2/MPP4; nuclear factor associated with dsRNA; double-stranded RNA-binding protein, 76 kD; nuclear factor of activated T-cells 90 kDa; nuclear factor of activated T-cells, 90 kD; CBTF; DRBF; MMP4; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; NF110b; NFAR-1; TCP110; NF-AT-90
• Gene ID: 3609
• mRNA Refseq: NM_012218
• Protein Refseq: NP_036350
• MIM: 603182
• UniProt ID: Q12906

Reviews

07/17/2022

The impurities are not obvious, clear and transparent, and the purity is high.

07/13/2022

It can meet the needs of daily experiments, and the expression effect is good.

02/27/2021

ILF3 has performed well in experiments and is a reliable choice.

Q&As

Which tumors are associated with ILF3? What role does it play in tumor development?

This is associated with a variety of tumors, including breast cancer, lung cancer, and colon cancer. In tumor development, ILF3 may promote cell proliferation, apoptosis inhibition, and metastasis.

What progress has been made in the exploration of new methods and new drugs for ILF3 treatment of tumors?

At present for ILF3 new treatment for cancer and new drug exploration is still in its infancy, but some ILF3 related drugs and treatment strategies are in development and evaluation, bring new hope for patients.

Is ILF3 associated with tumor microenvironment and immune escape?

This peotein may be associated with tumor microenvironment and immune escape, and can regulate the interaction between tumor cells and immune cells, further deepening our understanding of the mechanism of immune escape.

Is there a relationship between LF3 and tumor prognosis and treatment response?

The association between ILF3 and tumor prognosis and treatment response needs to be further studied, but according to the current study, high expression of ILF3 may be associated with poor prognosis and drug resistance in cancer patients.

What are the functions and mechanisms of ILF3 in infectious diseases?

ILF3 may play an important immunomodulatory function in infectious diseases and may be involved in the immune response and inflammatory response caused by viral or bacterial infection.

What is ILF3 role in autoimmune diseases? What autoimmune diseases might it be involved in?

It may play an important role in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (sle), may be involved in these diseases by means of immune regulation of occurrence and development.