Recombinant Human INSIG2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: INSIG2-2114H
• Product Overview: INSIG2 MS Standard C13 and N15-labeled recombinant protein (NP_057217) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 24.8 kDa
• AA Sequence: MAEGETESPGPKKCGPYISSVTSQS VNLMIRGVVLFFIGVFLALVLNLLQ IQRNVTLFPPDVIASIFSSAWWVPP CCGTASAVIGLLYPCIDRHLGEPHK FKREWSSVMRCVAVFVGINHASAKV DFDNNIQLSLTLAALSIGLWWTFDR SRSGFGLGVGIAFLATVVTQLLVYN GVYQYTSPDFLYVRSWLPCIFFAGG ITMGNIGRQLAMYECKVIAEKSHQE TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: INSIG2 insulin induced gene 2 [ Homo sapiens (human) ]
• Official Symbol: INSIG2
• Synonyms: INSIG2; insulin induced gene 2; INSIG-2; insulin-induced gene 2 protein; INSIG2 membrane protein; insulin induced protein 2
• Gene ID: 51141
• mRNA Refseq: NM_016133
• Protein Refseq: NP_057217
• MIM: 608660
• UniProt ID: Q9Y5U4

Reviews

08/16/2020

We observed that results obtained with the product remained stable and reliable over long-term experiments.

01/11/2020

The product's impact extended to data analysis, with intuitive tools for interpreting experimental results.

07/07/2017

Its adaptability to various sample types and conditions made it a valuable asset for our diverse projects.

Q&As

Are there any post-translational modifications known to regulate INSIG2?

While specific post-translational modifications may exist, the current literature provides limited information on post-translational regulation of INSIG2.

How does INSIG2 contribute to insulin sensitivity?

INSIG2 contributes to insulin sensitivity by regulating lipid metabolism and insulin signaling pathways.

How does INSIG2 impact the activity of SREBP-1 and SREBP-2?

INSIG2 inhibits the activity of both SREBP-1 and SREBP-2, impacting lipogenesis and cholesterol biosynthesis.

What is the relationship between INSIG2 and cholesterol synthesis?

INSIG2 is a key regulator of cholesterol synthesis, modulating the activation of SREBP-2, a master regulator of cholesterol biosynthesis.

What is the role of INSIG2 in cellular response to ER stress?

INSIG2 is involved in the cellular response to ER stress, playing a role in maintaining ER homeostasis.

Is there evidence of INSIG2 involvement in cancer development?

There is evidence linking INSIG2 to cancer development, suggesting a potential role in tumorigenesis.

Does INSIG2 expression vary in response to different dietary conditions?

Yes, INSIG2 expression can vary in response to different dietary conditions, reflecting its role in metabolic adaptation.