Recombinant Human IL10RB protein(Met20-Ser220), His-hFc-tagged
Cat.No. : | IL10RB-1647H |
Product Overview : | Recombinant NS0-derived Human IL10RB protein(Met20-Ser220)(Q08334), fused with C-terminal His and hFc tag, was expressed in Mouse myeloma cell line. |
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Source : | Mouse myeloma cell line |
Species : | Human |
Tag : | C-His-hFc |
Protein length : | Met20-Ser220 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human IL 28 R alpha /IFN lambda R1 Fc Chimera is immobilized at 5.00 μg/mL (100 μL/well), the concentration of Recombinant Human IL-10 R beta Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.300-3.00 μg/mL. |
Molecular Mass : | 75-85 kDa, reducing conditions |
Endotoxin : | <0.01 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 100 μg/mL in sterile PBS. |
Gene Name : | IL10RB interleukin 10 receptor, beta [ Homo sapiens ] |
Official Symbol : | IL10RB |
Synonyms : | IL10RB; interleukin 10 receptor, beta; CRFB4, D21S58, D21S66; interleukin-10 receptor subunit beta; CDW210B; CRF2 4; IL 10R2; IL-10RB; IL-10R subunit 2; IL-10R subunit beta; IL-10 receptor subunit beta; cytokine receptor class-II CRF2-4; interleukin-10 receptor subunit 2; cytokine receptor class-II member 4; cytokine receptor family 2 member 4; cytokine receptor family II, member 4; CRFB4; CRF2-4; D21S58; D21S66; IL-10R2; |
Gene ID : | 3588 |
mRNA Refseq : | NM_000628 |
Protein Refseq : | NP_000619 |
MIM : | 123889 |
UniProt ID : | Q08334 |
Products Types
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◆ Lysates | ||
IL10RB-2677HCL | Recombinant Human IL10RB cell lysate | +Inquiry |
IL10RB-1371RCL | Recombinant Rat IL10RB cell lysate | +Inquiry |
IL10RB-1795MCL | Recombinant Mouse IL10RB cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionMass spectrometry-based interactomics identifies proteins interacting with IL10RB.
NMR spectroscopy helps understand dynamic interactions and structural shifts in IL10RB upon binding.
Proximity labeling and quantitative proteomics show IL10RB's changing protein network with IL-10.
Techniques like surface plasmon resonance and isothermal titration calorimetry measure IL10RB-IL-10 binding strength and kinetics.
Flow cytometry and single-cell transcriptomics reveal IL10RB's expression diversity in cells and immune subsets.
Mutagenesis and alanine scanning pinpoint amino acids essential for IL10RB's binding to IL-10.
CRISPR-edited cells and knockout models explore IL10RB's signaling after IL-10 binding.
X-ray crystallography and cryo-electron microscopy provide structural insights into IL10RB's changes upon IL-10 interaction.
Genome-wide CRISPR screening uncovers genes affecting IL10RB expression and function.
Co-immunoprecipitation and FRET study protein complexes involving IL10RB and IL-10.
Customer Reviews (3)
Write a reviewReliable in co-immunoprecipitation.
High specificity.
Effective in signaling pathways.
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