Recombinant Human Interleukin 12 Receptor, Beta 1

• Cat.No.: IL12RB1-1649H

Specification

• Cat. No.: IL12RB1-1649H
• Description: Interleukin 12 receptor, beta 1 (IL12RB1) also known as CD212 (cluster of differentiation 212) is a subunit of the interleukin 12 receptor. IL12RB1 is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. IL-12 Rβ1 defect is generally associated with selective susceptibility to weakly pathogenic mycobacteria and Salmonella species.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human IL-12 Rβ1 is Mr 84.5 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 110 kDa.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: IL12RB1 interleukin 12 receptor, beta 1 [ Homo sapiens ]
• Synonyms: interleukin 12 receptor, beta 1; CD212; IL12RB; MGC34454; IL-12R-BETA1; IL12RB1; IL-12 receptor beta component; interleukin-12 receptor beta-1 chain; IL-12R-beta-1; IL-12RB1; IL12R; CD212 antigen; Interleukin-12 receptor beta
• Gene ID: 3594
• mRNA Refseq: NM_005535
• Protein Refseq: NP_005526
• MIM: 601604
• UniProt ID: P42701
• Chromosome Location: 19p13.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine receptor activity

Reviews

04/06/2022

Reliable in co-immunoprecipitation.

10/18/2021

Effective in asthma models.

12/21/2017

great in Western blot analysis.

Q&As

Could you explain the significance of CRISPR/Cas9-mediated gene tagging in tracking IL12RB1 localization?

Gene tagging allows real-time tracking of IL12RB1 subcellular localization.

Can you elaborate on the use of RNA-seq analysis to profile gene expression changes upon IL12RB1 activation?

RNA-seq profiles gene expression changes upon IL12RB1 activation.

What are the applications of ChIP-seq in exploring IL12RB1's role in gene regulation?

ChIP-seq investigates IL12RB1's impact on gene regulation networks.

What methods can be employed to assess IL12RB1 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation assess IL12RB1 dimerization and signaling.

What techniques are suitable for studying IL12RB1's interaction with IL12A and IL23A?

Co-immunoprecipitation and ELISA assay IL12RB1 interactions with cytokines.

How can the role of IL12RB1 in signal transduction be elucidated using knockdown experiments?

Knockdown experiments reveal IL12RB1's contribution to signal transduction.

What are the advantages of using siRNA screening to identify IL12RB1-regulated pathways?

siRNA screening identifies pathways influenced by IL12RB1 expression.

How do co-crystallization studies aid in understanding the molecular details of IL12RB1 interactions?

Co-crystallization offers structural insights into IL12RB1 interactions.

How does flow cytometry provide insights into IL12RB1 expression levels on different cell types?

Flow cytometry quantifies IL12RB1 levels on diverse cell populations.

What experimental approaches validate IL12RB1's involvement in cytokine-mediated immune responses?

Functional assays validate IL12RB1's role in immune responses.