Recombinant Human Interleukin 18 Receptor Accessory Protein, Fc-His

• Cat.No.: IL18RAP-2255H
• Product Overview: Recombinant Human Interleukin 18 Receptor Accessory encoding the human IL18RAP (Met 1-Arg 356) was fused with the His-tagged Fc region of human IgG1 at the C- terminus. It is expressed inHuman Cells.

Specification

• Cat. No.: IL18RAP-2255H
• Description: Interleukin 18 receptor accessory protein, also known as IL18RAP, IL1R7 and CDw218b, is a single-pass type I membrane protein. It is member of the interleukin-1 receptor family. IL18RAP contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. IL18RAP is strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon. It is not expressed in any other tissue tested. IL18RAP is also strongly expressed in T-cells polarized with IL12 and specifically coexpressed with IL18R1 in Th1 cells.
• Source: Human Cells.
• Purity: >95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70⁰C.
• Predicted N Terminal: Phe 20.
• Molecular Mass: The recombinant human IL18RAP /Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 575 amino acids and has a calculated molecular mass of 65.2 KDa. As a result of glycosylation, the apparent molecular mass of rhIL18RAP/Fc monomer is approximately 80 -90 KDa in reduced SDS-PAGE.
• Formulation: Supplied as a 0.2μm filtered solution of PBS, pH7.4.
• Reconstitution: Follow the instructions on the vial. Centrifuge the vial at 4⁰C before opening to recover the entire contents.
• Storage: Store it under sterile conditions at -70⁰C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL18RAP interleukin 18 receptor accessory protein [ Homo sapiens ]
• Synonyms: IL18RAP; interleukin 18 receptor accessory protein; ACPL; CD218b; IL18RB; CDw218b; MGC120589; MGC120590; IL-18 receptor beta; accessory protein-like; AcPL; IL-18RAcP; IL-18Rbeta; IL-1R7; IL-1RAcPL; OTTHUMP00000161337; CD218 antigen-like family member B; CD218b antigen; IL-18 receptor accessory protein; IL-1R accessory protein-like
• Gene ID: 8807
• mRNA Refseq: NM_003853
• Protein Refseq: NP_003844
• MIM: 604509
• UniProt ID: O95256
• Chromosome Location: 2q12
• Pathway: Cytokine-cytokine receptor interaction
• Function: transmembrane receptor activity

Reviews

02/09/2021

Effective in signaling studies.

11/17/2020

High specificity.

09/16/2018

Reliable in co-immunoprecipitation.

Q&As

What experimental methods are commonly employed to study the expression patterns and regulation of IL18RAP across different cell types and conditions?

RT-qPCR, immunohistochemistry, and single-cell RNA sequencing characterize IL18RAP expression levels and regulation in diverse cellular contexts.

What techniques are utilized to investigate the role of IL18RAP in immune responses, inflammation, and diseases, and how do researchers assess its potential as a therapeutic target?

Animal models, patient samples, and therapeutic interventions elucidate IL18RAP's involvement in immune-related disorders and its potential as a therapeutic target.

Could you provide insights into the experimental approaches used to explore the functional effects of IL18RAP isoforms and their specific contributions to IL18 signaling?

Isoform-specific knockdown, mutagenesis, and functional assays delineate IL18RAP isoforms' distinct roles in modulating IL18-mediated responses.

How are co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and biophysical assays applied to characterize the interactions between IL18RAP, IL18, and IL18R1?

Co-immunoprecipitation assays, FRET studies, and biophysical techniques characterize IL18RAP's interactions with IL18 and IL18R1 partners.

What experimental methods are used to elucidate the effects of post-translational modifications, such as glycosylation, on IL18RAP's structure and function?

Mass spectrometry, mutagenesis, and functional assays investigate the impact of post-translational modifications on IL18RAP's conformation and function.

How do researchers experimentally determine the binding affinity and kinetics of IL18RAP to IL18 and IL18R1, considering its pivotal role in forming the IL18 receptor complex?

Surface plasmon resonance, bio-layer interferometry, and surface plasmon-enhanced fluorescence assays quantify IL18RAP's binding kinetics to IL18 and IL18R1.

Can you explain the use of knockdown, knockout, and gene editing techniques to uncover the physiological importance of IL18RAP in mediating IL18 signaling?

siRNA knockdown, CRISPR/Cas9-mediated knockout, and transgenic models unravel IL18RAP's physiological roles and contributions to IL18 signaling.

How are advanced imaging techniques, such as single-molecule imaging and super-resolution microscopy, utilized to visualize the dynamic assembly of the IL18 receptor complex involving IL18RAP?

Single-molecule imaging, super-resolution microscopy, and live-cell imaging visualize the dynamic assembly of IL18 receptor complexes involving IL18RAP.

Can you elaborate on the strategies used to investigate the structural features of IL18RAP's interaction interfaces with IL18 and IL18R1, and how these interfaces contribute to receptor activation?

X-ray crystallography, NMR spectroscopy, and computational modeling reveal IL18RAP's structural interactions with IL18 and IL18R1 domains.

How do researchers dissect the intracellular signaling pathways triggered by IL18RAP upon binding to IL18 and its collaboration with IL18R1?

Phosphoprotein analysis, pathway-specific inhibitors, and functional assays dissect IL18RAP's intracellular signaling cascades and its crosstalk with IL18R1.