Recombinant Human Interleukin 32

• Cat.No.: IL32-301H
• Product Overview: Recombinant Human IL-32 alpha produced in E.coli is a 131 amino acid non-glycosylated polypeptide with a molecular mass of 14.9 kDa.MW = 14.9 kDa.

Specification

• Cat. No.: IL32-301H
• Description: Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-α, and MIP-2 production from human & mouse macrophage cell lines. It is up-regulated in activated T- & NKcells, and IFN-γ-treated epithelial cells.
• Source: E.coli.
• Purity: Greater than 97% (determined by SDS-PAGE).
• Form: Lyophilized from a protein solution containing 50 mM NaP, pH 7.5.
• Endotoxin Level: < 0.1 ng per μg of rHu IL-32 alpha (1EU/μg).
• Specific Activity: NA.
• Biological Activity: Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED₅₀for this assay is in the range of 0.125-1.0 μg/ml.
• Reconstitution: Please Note: Always centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
• Storage & Stability: The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu IL-32 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: IL32 interleukin 32 [ Homo sapiens ]
• Synonyms: IL32; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor; Interleukin-32; IL-32
• Gene ID: 9235
• mRNA Refseq: NM_001012631
• Protein Refseq: NP_001012649
• MIM: 606001
• UniProt ID: P24001
• Chromosome Location: 16p13.3
• Function: cytokine activity

Reviews

12/13/2022

IL32 protein has a long shelf life, which facilitates the continuity of subsequent experiments.

05/20/2022

Consistent results can be obtained using IL32 experiments and experimental uncertainty can be reduced.

10/26/2019

The IL32 shows excellent stability and repeatability under specific experimental conditions.

Q&As

Can recombinant IL32 be used to treat autoimmune diseases?

At present, research is ongoing, and IL32 is considered to have the potential to treat autoimmune diseases, but further clinical validation is required.

What is IL32?

IL32, a protein of human origin, is an interferon-induced cytokine with a variety of biological functions.

What is the progress of IL32 in clinical trials?

IL32 is in clinical trials and will take time to evaluate its safety and efficacy.

Is recombinant IL32 associated with inflammation?

IL32 induce and regulate inflammatory response and participate in immune and inflammatory processes.

Can IL32 improve immunity?

Can enhance the activity and function of immune cells, thereby improving the body immunity.

What are the side effects of IL32?

So far, there have been no clear reports of side effects of IL32, but attention should be paid to reasonable dosage and method of use when using it.