Recombinant Human LEPR protein(Met1-Asp839), His&hFc-tagged

• Cat.No.: LEPR-800H
• Product Overview: Recombinant Human leptin receptor (NP_002294.2)(Met 1-Asp 839) was expressed in HEK293 with the C-terminal fused polyhistidine-tagged Fc region of Human IgG1.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His&hFc
• Protein length: Met1-Asp839
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032-4.0 μg/ml.
• Molecular Mass: The recombinant human LEPR/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 1065 amino acids and has a predicted molecular mass of 121.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LEPR/Fc monomer is approximately 155-165 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LEPR leptin receptor [ Homo sapiens ]
• Official Symbol: LEPR
• Synonyms: LEPR; leptin receptor; CD295; OBR; huB219; OB receptor; OB-R; LEP-R; DKFZp686B1731
• Gene ID: 3953
• mRNA Refseq: NM_001003679
• Protein Refseq: NP_001003679
• UniProt ID: P48357