| Description : |
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
81.1 kDa |
| AA Sequence : |
MALKDYALEKEKVKKFLQEFYQDDE LGKKQFKYGNQLVRLAHREQVALYV DLDDVAEDDPELVDSICENARRYAK LFADAVQELLPQYKEREVVNKDVLD VYIEHRLMMEQRSRDPGMVRSPQNQ YPAELMRRFELYFQGPSSNKPRVIR EVRADSVGKLVTVRGIVTRVSEVKP KMVVATYTCDQCGAETYQPIQSPTF MPLIMCPSQECQTNRSGGRLYLQTR GSRFIKFQEMKMQEHSDQVPVGNIP RSITVLVEGENTRIAQPGDHVSVTG IFLPILRTGFRQVVQGLLSETYLEA HRIVKMNKSEDDESGAGELTREELR QIAEEDFYEKLAASIAPEIYGHEDV KKALLLLLVGGVDQSPRGMKIRGNI NICLMGDPGVAKSQLLSYIDRLAPR SQYTTGRGSSGVGLTAAVLRDSVSG ELTLEGGALVLADQGVCCIDEFDKM AEADRTAIHEVMEQQTISIAKAGIL TTLNARCSILAAANPAYGRYNPRRS LEQNIQLPAALLSRFDLLWLIQDRP DRDNDLRLAQHITYVHQHSRQPPSQ FEPLDMKLMRRYIAMCREKQPMVPE SLADYITAAYVEMRREAWASKDATY TSARTLLAILRLSTALARLRMVDVV EKEDVNEAIRLMEMSKDSLLGDKGQ TARTQRPADVIFATVRELVSGGRSV RFSEAEQRCVSRGFTPAQFQAALDE YEELNVWQVNASRTRITFVTRTRPL EQKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
