Recombinant Human MCT1 protein, His-tagged

• Cat.No.: MCT1-3816H
• Product Overview: Recombinant Human MCT1 protein(451-500 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 451-500 aa
• AA Sequence: EQKANEQKKESKEEETSIDVAGKPN EVTKAAESPDQKDTDGGPKEEESPV
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Reviews

12/02/2021

Their prompt assistance and prompt response can swiftly resolve any challenges, enabling smooth experimentation and maintaining the integrity of the results.

09/01/2021

They keep up with the latest research findings and continually refine their product based on customer feedback and emerging scientific trends.

06/24/2017

Their knowledgeable support team is well-equipped to address specific inquiries, suggest experimental protocols, and provide guidance throughout the entire research process.

Q&As

Are there any genetic disorders associated with MCT1 protein?

Yes, mutations in the SLC16A1 gene that encodes MCT1 protein have been associated with certain metabolic disorders, such as exercise-induced hyperinsulinism.

What methods are used to study MCT1 protein in clinical research?

Various techniques such as immunohistochemistry, western blotting, and gene expression analysis are used to study the expression and activity of MCT1 protein in clinical research.

Can MCT1 protein impact exercise performance?

Yes, MCT1 is involved in lactate transport in muscle cells, which can affect exercise performance and endurance.

How is MCT1 protein involved in cancer metabolism?

MCT1 facilitates the uptake of lactate by cancer cells, which can then be used as an energy source. This is known as the lactate shuttle hypothesis and has implications for cancer growth and progression.

Are there any clinical implications of MCT1 protein in cancer treatment?

Yes, targeting MCT1 protein has been explored as a potential therapeutic strategy for cancer treatment, particularly in combination with other targeted therapies.