Recombinant Human MLH1, StrepII-tagged

• Cat.No.: MLH1-233H
• Product Overview: Purified human recombinant DNA mismatch repair MLH1 protein (amino acids 410-652, 243 a.a.) with StrepII tag, produced in human cells. Predicted molecular weight: 27.4 kDa. (Accession NP_000240.1; UniProt P40692)

Specification

• Description: This product is a fragment of MLH1, which is a component of the post-replicative DNA mismatch repair system (MMR). It is a human homolog of the E. coli DNA mismatch repair gene mutL. Native MLH1 heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
• Source: Human Cells
• Species: Human
• Tag: StrepII
• Form: Lyophilized from sterile 0.2μm filtered solution in 0.3X PBS with 10% Trehalose (carrier-free).
• Protein length: 410-652, 243 a.a.
• AA Sequence: AIVTEDKTDISSGRARQQDEEMLEL PAPAEVAAKNQSLEGDTTKGTSEMS EKRGPTSSNPRKRHREDSDVEMVED DSRKEMTAACTPRRRIINLTSVLSL QEEINEQGHEVLREMLHNHSFVGCV NPQWALAQHQTKLYLLNTTKLSEEL FYQILIYDFANFGVLRLSEPAPLFD LAMLALDSPESGWTEEDGPKEGLAE YIVEFLKKKAEMLADYFSLEIDEEG NLIGLPLLIDNYVPPLEG
• Endotoxin: <0.1 eu per ug protein by lal
• Purity: >95% pure by SDS-PAGE
• Storage: 12 months at -20°C as supplied; 1 month at 4°C after reconstitution. Avoid freeze/thaw cycles.
• Reconstitution: Centrifuge the vial prior to opening. Reconstitute in PBS to a concentration of 0.1 mg/ml.

Gene Information

• Gene Name: MLH1 mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) [ Homo sapiens ]
• Official Symbol: MLH1
• Synonyms: MLH1; mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); COCA2, mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2); DNA mismatch repair protein Mlh1; FCC2; HNPCC; HNPCC2; COCA2; hMLH1; MGC5172
• Gene ID: 4292
• mRNA Refseq: NM_000249
• Protein Refseq: NP_000240
• MIM: 120436
• UniProt ID: P40692
• Chromosome Location: 3p22.3
• Pathway: BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; Colorectal cancer, organism-specific biosystem; Colorectal cancer, conserved biosystem; Direct p53 effectors, organism-specific biosystem; Endometrial cancer, organism-specific biosystem; Endometrial cancer, conserved biosystem; Fanconi anemia pathway, organism-specific biosystem
• Function: ATP binding; ATPase activity; contributes_to MutSalpha complex binding; guanine/thymine mispair binding; mismatched DNA binding; protein binding; contributes_to protein binding; contributes_to single-stranded DNA binding

Reviews

11/23/2022

Timely protein expression optimization, greatly boosts our research.

04/22/2022

Accurate protein structural analysis, a cornerstone of our investigations.

10/01/2020

Precise antibody validation, enhances our immunoprecipitation accuracy.

Q&As

What is the impact of germline mutations in MLH1 on Lynch syndrome and associated cancer risks?

Germline mutations in MLH1 are linked to Lynch syndrome, increasing the risk of various cancers.

What role does MLH1 play in the prevention of colorectal and other hereditary cancers?

MLH1 helps prevent the development of colorectal and other hereditary cancers by maintaining DNA integrity.

How do alterations in MLH1 expression or function affect chemotherapy efficacy in cancer treatment?

Changes in MLH1 function can influence the effectiveness of certain chemotherapies in treating cancer.

How does MLH1 influence the cellular response to DNA damage and the maintenance of replication fidelity?

MLH1 plays a role in the cellular response to DNA damage, maintaining replication accuracy.

How does MLH1 deficiency lead to microsatellite instability in cancer cells?

MLH1 deficiency results in microsatellite instability, a hallmark of certain types of cancer.

How does MLH1 (MutL Homolog 1) contribute to DNA mismatch repair and genomic stability?

MLH1 is essential for DNA mismatch repair, ensuring genomic stability by correcting replication errors.

What are the mechanisms by which MLH1 modulates apoptosis in response to DNA damage?

MLH1 modulates apoptosis in cells with DNA damage, preventing the proliferation of damaged cells.