Recombinant Human NCR2 cell lysate
Cat.No. : | NCR2-2109HCL |
Product Overview : | Human NKp44 / NCR2 / CD336 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
- Specification
- Gene Information
- Related Products
- Download
Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | NCR2 natural cytotoxicity triggering receptor 2 [ Homo sapiens ] |
Official Symbol : | NCR2 |
Synonyms : | NCR2; natural cytotoxicity triggering receptor 2; LY95, lymphocyte antigen 95 (activating NK receptor; NK p44); CD336; NK p44; NK cell-activating receptor; NK cell activating receptor (NKp44); natural killer cell p44-related protein; lymphocyte antigen 95 (activating NK-receptor; NK-p44); lymphocyte antigen 95 homolog (activating NK-receptor; LY95; NKP44; NK-p44; dJ149M18.1; |
Gene ID : | 9436 |
mRNA Refseq : | NM_001199509 |
Protein Refseq : | NP_001186438 |
MIM : | 604531 |
UniProt ID : | O95944 |
Chromosome Location : | 6p21.1 |
Pathway : | Natural killer cell mediated cytotoxicity, organism-specific biosystem; Natural killer cell mediated cytotoxicity, conserved biosystem; |
Function : | receptor activity; transmembrane signaling receptor activity; |
Products Types
◆ Recombinant Protein | ||
NCR2-1134H | Recombinant Human NCR2 Protein (Met1-Pro190), His-tagged, Biotinylated | +Inquiry |
NCR2-125H | Recombinant Human NCR2 Protein, His (Fc)-Avi-tagged | +Inquiry |
NCR2-236H | Recombinant Human NCR2 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
NCR2-473H | Recombinant Human NCR2 Protein, MYC/DDK-tagged | +Inquiry |
NCR2-188H | Recombinant Human NCR2 Protein, C-His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (3)
Ask a questionNCR2 is only expressed on the surface of activated natural killer (NK) cells.
NCR2 interacts with DAP12.
NCR2 proteins function as cytotoxic activation receptors that enhance the efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Customer Reviews (3)
Write a reviewThe NCR2 proteins purchased from your company is suitable for ELISA.
The technical expert communicated with me in time to understand the progress of my experiment and gave me suggestions for improvement.
The high quality of the purchased protein helped me to obtain satisfactory experimental results.
Ask a Question for All NCR2 Products
Required fields are marked with *
My Review for All NCR2 Products
Required fields are marked with *
Inquiry Basket