Recombinant Human NEDD4 protein, His-tagged

• Cat.No.: NEDD4-2461H
• Product Overview: Recombinant Human NEDD4 protein(970-1319 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 970-1319 aa
• AA Sequence: TVLEDSYRRIMGVKRADFLKARLWI EFDGEKGLDYGGVAREWFFLISKEM FNPYYGLFEYSATDNYTLQINPNSG LCNEDHLSYFKFIGRVAGMAVYHGK LLDGFFIRPFYKMMLHKPITLHDME SVDSEYYNSLRWILENDPTELDLRF IIDEELFGQTHQHELKNGGSEIVVT NKNKKEYIYLVIQWRFVNRIQKQMA AFKEGFFELIPQDLIKIFDENELEL LMCGLGDVDVNDWREHTKYKNGYSA NHQVIQWFWKAVLMMDSEKRIRLLQ FVTGTSRVPMNGFAELYGSNGPQSF TVEQWGTPEKLPRAHTCFNRLDLPP YESFEELWDKLQMAIENTQGFDGVD
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: NEDD4 neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase [ Homo sapiens ]
• Official Symbol: NEDD4
• Synonyms: NEDD4; neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase; neural precursor cell expressed, developmentally down regulated 4; E3 ubiquitin-protein ligase NEDD4; KIAA0093; MGC176705; NEDD4 1; receptor potentiating factor 1; RPF1; NEDD-4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4; NEDD4-1
• Gene ID: 4734
• mRNA Refseq: NM_006154
• Protein Refseq: NP_006145
• MIM: 602278
• UniProt ID: P46934