Recombinant Human NSUN6 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NSUN6-2817H
• Product Overview: NSUN6 MS Standard C13 and N15-labeled recombinant protein (NP_872349) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 51.8 kDa
• AA Sequence: MSIFPKISLRPEVENYLKEGFMNKE IVTALGKQEAERKFETLLKHLSHPP SFTTVRVNTHLASVQHVKNLLLDEL QKQFNGLSVPILQHPDLQDVLLIPV IGPRKNIKKQQCEAIVGAQCGNAVL RGAHVYAPGIVSASQFMKAGDVISV YSDIKGKCKKGAKEFDGTKVFLGNG ISELSRKEIFSGLPELKGMGIRMTE PVYLSPSFDSVLPRYLFLQNLPSAL VSHVLNPQPGEKILDLCAAPGGKTT HIAALMHDQGEVIALDKIFNKVEKI KQNALLLGLNSIRAFCFDGTKAVKL DMVEDTEGEPPFLPESFDRILLDAP CSGMGQRPNMACTWSVKEVASYQPL QRKLFTAAVQLLKPEGVLVYSTCTI TLAENEEQVAWALTKFPCLQLQPQE PQIGGEGMRGAGLSCEQLKQLQRFD PSAVPLPDTDMDSLREARREDMLRL ANKDSIGFFIAKFVKCKSTTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NSUN6 NOP2/Sun RNA methyltransferase 6 [ Homo sapiens (human) ]
• Official Symbol: NSUN6
• Synonyms: NSUN6; NOP2/Sun domain family, member 6; NOL1/NOP2/Sun domain family 6, NOL1/NOP2/Sun domain family, member 6, NOPD1; putative methyltransferase NSUN6; FLJ23743; NOL1/NOP2/Sun domain family 6; NOL1/NOP2/Sun domain family member 6; NOL1/NOP2/Sun domain family, member 6; NOL1/NOP2/Sun and PUA domain-containing protein 1; nucleolar protein (NOL1/NOP2/sun) and PUA domains 1; NOPD1; 4933414E04Rik
• Gene ID: 221078
• mRNA Refseq: NM_182543
• Protein Refseq: NP_872349
• MIM: 617199
• UniProt ID: Q8TEA1