Recombinant Human Pancreatic And Duodenal Homeobox 1

• Cat.No.: PDX1-176H
• Product Overview: Recombinant Human Insulin promoter factor-1 Protein, produced inE. coli, contains 283 amino acids and has a molecular mass of 30.64 kDa.

Specification

• Cat. No.: PDX1-176H
• Description: Pancreatic Duodenal Homeobox-1 activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. binds preferentially the dna motif 5"-[ct]taat[tg]-3". During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. at adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus.
• Source: Escherichia Coli.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Formulation: Lyophilized Powder of liquid in PBS, pH 7.4.
• Purity: Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Solubility: It is recommended to reconstitute the lyophilized PDX1 in sterile PBS, 10% glycerol to prepare a stock of 100μg/ml.
• Biological Acivity: The activity of human PDX-1 is determined by the ability to stimulate NeuroD1 gene expression in WB cells and binding to insulin promoter be EMSA assay.
• Stability: Recombinant Human PDX1 although stable at 25°C 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PDX1 pancreatic and duodenal homeobox 1 [ Homo sapiens ]
• Synonyms: GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1; PDX1; IUF-1; IPF-1;Pancreas/duodenum homeobox protein 1; Insulin promoter factor 1; Islet/duodenum homeobox-1; Somatostatin-transactivating factor 1; Insulin upstream factor 1; Glucose-sensitive factor; pancreatic and duodenal homeobox 1; glucose-sensitive factor; insulin upstream factor 1; somatostatin transcription factor 1; pancreatic-duodenal homeobox factor 1; homeodomain transcription factor
• Gene ID: 3651
• mRNA Refseq: NM_000209
• Protein Refseq: NP_000200
• MIM: 600733
• UniProt ID: P52945
• Chromosome Location: 13q12.1
• Pathway: Maturity onset diabetes of the young; Type II diabetes mellitus; Regulation of beta-cell development
• Function: protein binding; sequence-specific DNA binding; specific RNA polymerase II transcription factor activity; transcription activator activity; transcription factor activity

Reviews

08/03/2022

The expression was better at high humidity.

06/18/2022

It is stable in clinical trials.

06/01/2021

It has stable expression in signal transduction.

Q&As

How to study the regulatory mechanism of PDX1?

Studying the regulatory mechanism of PDX1 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

Do abnormal PDX1 levels suggest some disease?

Abnormal PDX1 levels may indicate pancreas-related diseases such as diabetes, pancreatitis, and pancreatic cancer.

How are mutations in PDX1 detected and analyzed?

Mutations in PDX1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of PDX1?

The types of mutations in PDX1 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

Is there a relationship between PDX1 and other genes or proteins?

PDX1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.

What are the applications of PDX1 in medical treatment?

It can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting PDX1, such as gene therapy or modulating its expression, are also being studied.