Recombinant Mouse Pdx1 Protein, Myc/DDK-tagged

• Cat.No.: Pdx1-4780M
• Product Overview: Purified recombinant protein of mouse full-length pancreatic and duodenal homeobox 1 (Pdx1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CC[CT]TAATGGG-3'.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 31.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pdx1 pancreatic and duodenal homeobox 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pdx1
• Synonyms: PDX1; pancreatic and duodenal homeobox 1; pancreas/duodenum homeobox protein 1; islet/duodenum homeobox 1; somatostatin-transactivating factor 1; pancreatic and duodenal homeobox gene 1; insulin promoter factor 1, homeodomain transcription factor; Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1
• Gene ID: 18609
• mRNA Refseq: NM_008814
• Protein Refseq: NP_032840
• UniProt ID: P52946