Recombinant Human PDGFB Protein

• Cat.No.: PDGFB-33H
• Product Overview: Recombinant human PDGF-BB protein without tag was expressed in E. coli.
  Animal-free (AOF) and carrier protein-free.
  Mass spectrometry: single species with expected mass.
  Analytical reversed-phase: single sharp peak.
  Recovery from stock vial: >95%

Specification

• Description: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Molecular Mass: 25 kDa
• Endotoxin: <0.005 EU/μg protein (below level of detection)
• Applications: Differentiation of human pluripotent Stem cell (hPSC)-derived neural progenitor cells into oligodendrocyte precursor cells
• Storage: Resuspend in water at >100 μg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade
• Storage Buffer: Lyophilized from acetonitrile, TFA

Gene Information

• Gene Name: PDGFB platelet derived growth factor subunit B [ Homo sapiens (human) ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet derived growth factor subunit B; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2; platelet-derived growth factor subunit B; PDGF subunit B; PDGF, B chain; becaplermin; epididymis secretory sperm binding protein; platelet-derived growth factor 2; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); platelet-derived growth factor, beta polypeptide (oncogene SIS); proto-oncogene c-Sis
• Gene ID: 5155
• mRNA Refseq: NM_002608
• Protein Refseq: NP_002599
• MIM: 190040
• UniProt ID: P01127