Recombinant Human PDX1 protein, Arginine-tagged

• Cat.No.: PDX1-147H
• Product Overview: Recombinant human PDX1 protein fused with 11 arginine domain at C-terminal, which efficiently delivery protein intracellularly, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: Arginine
• Form: 0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
• AA Sequence: MNGEEQYYAATQLYKDPCAFQRGPA PEFSASPPACLYMGRQPPPPPPHPF PGALGALEQGSPPDISPYEVPPLAD DPAVAHLHHHLPAQLALPHPPAGPF PEGAEPGVLEEPNRVQLPFPWMKST KAHAWKGQWAGGAYAAEPEENKRTR TAYTRAQLLELEKEFLFNKYISRPR RVELAVMLNLTERHIKIWFQNRRMK WKKEEDKKRGGGTAVGGGGVAEPEQ DCAVTSGEELLALPPPPPPGGAVPP AAPVAAREGRLPPGLSASPQPSSVA PRRPQEPRESGGGGSPQRRRRRRRR RRR
• Purity: >90% by SDS-PAGE
• Applications: 1. Protein transduction for pancreatic β-cells in vitro differentiation.2. Active recombinant protein, may be used for ELISA based DNA/Protein binding assay.3. As specific protein substrate for kinase assay.
• Storage: Keep at -20°C for long term storage. Product is stable at 4 °C for at least 7 days.

Gene Information

• Gene Name: PDX1 pancreatic and duodenal homeobox 1 [ Homo sapiens ]
• Official Symbol: PDX1
• Synonyms: PDX1; IDX 1; MODY4; PDX 1; somatostatin transcription factor 1; STF 1; IPF-1; IUF-1; glucose-sensitive factor; insulin upstream factor 1; islet/duodenum homeobox-1; GSF; IPF1; IUF1; IDX-1; PDX-1; STF-1
• Gene ID: 3651
• mRNA Refseq: NM_000209
• Protein Refseq: NP_000200
• MIM: 600733
• UniProt ID: P52945
• Chromosome Location: 13q12.1
• Pathway: Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Maturity onset diabetes of the young, organism-specific biosystem; Maturity onset diabetes of the young, conserved biosystem; Regulation of beta-cell development, organism-specific biosystem; Regulation of gene expression in beta cells, organism-specific biosystem; Regulation of gene expression in early pancreatic precursor cells, organism-specific biosystem
• Function: chromatin binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity

Reviews

08/03/2022

The expression was better at high humidity.

06/18/2022

It is stable in clinical trials.

06/01/2021

It has stable expression in signal transduction.

Q&As

How to study the regulatory mechanism of PDX1?

Studying the regulatory mechanism of PDX1 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

Do abnormal PDX1 levels suggest some disease?

Abnormal PDX1 levels may indicate pancreas-related diseases such as diabetes, pancreatitis, and pancreatic cancer.

How are mutations in PDX1 detected and analyzed?

Mutations in PDX1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of PDX1?

The types of mutations in PDX1 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

Is there a relationship between PDX1 and other genes or proteins?

PDX1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.

What are the applications of PDX1 in medical treatment?

It can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting PDX1, such as gene therapy or modulating its expression, are also being studied.