Recombinant Human PICALM Protein, His-tagged

• Cat.No.: PICALM-2589H
• Product Overview: Recombinant Human PICALM Protein (Gly19-Leu285) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.2 kDa
  Accurate Molecular Mass: 34 kDa
• Protein length: Gly19-Leu285
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: PICALM phosphatidylinositol binding clathrin assembly protein [ Homo sapiens (human) ]
• Official Symbol: PICALM
• Synonyms: PICALM; phosphatidylinositol binding clathrin assembly protein; LAP; CALM; CLTH; phosphatidylinositol-binding clathrin assembly protein
  clathrin assembly lymphoid myeloid leukemia protein
• Gene ID: 8301
• mRNA Refseq: NM_007166
• Protein Refseq: NP_009097
• MIM: 603025
• UniProt ID: Q13492