Recombinant Human Poly (ADP-ribose) Polymerase 1
Cat.No. : | PARP1-1658H |
Product Overview : | Recombinant human PARP1 expressed by Baculovirus is a 116.0 kDa homodimeric protein. |
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Cat. No. : | PARP1-1658H |
Description : | PARP1 also known as NAD ADP-ribosyltransferase 1 or poly [ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 Enzyme, is highly purified and enzymatically active human PARP1, expressed in a baculovirus expression system. Upon addition of NAD+ and cofactors the PARP1 is automodified by the addition of ADP-ribose monomers to form poly (ADP-ribose) polymer. |
Biological Activity : | 1000 U/vial, specific activity = 20000 U/mg PARP1. 1U =10 fmol ADP-ribose incorporated into 5 μg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values. |
Molecular Weight : | 116.0 kDa |
Source : | Baculovirus |
Species : | Human |
Form : | Lyophilized solid. |
Appearance : | Liquid |
Reconstitution : | Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 μL dH₂O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 μL dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers. |
Purity : | ≥95% by SDS-PAGE |
Storage : | Store reconstituted solution at -70°C. Avoid multiple freeze-thaw cycles. There is loss of PARP enzymatic activity upon each free/thaw cycle. It is suggested to aliquot the reconstituted enzyme into multiple tubes and freeze at -70°C. Alternatively, add glycerol at 1:1 vol/vol to the reconstituted PARP1, mix gently by trituration, and store at -20°C (do not store in a frost-free freezer!) for up to 6 months. |
Gene Name : | PARP1 poly (ADP-ribose) polymerase 1 [ Homo sapiens ] |
Official Symbol : | PARP1 |
Synonyms : | PARP1; poly (ADP-ribose) polymerase 1; PARP; PPOL; ADPRT; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; poly [ADP-ribose] polymerase 1; OTTHUMP00000035663; poly(ADP-ribose) polymerase; poly(ADP-ribose) synthetase; poly[ADP-ribose] synthase 1; poly(ADP-ribosyl)transferase; ADP-ribosyltransferase NAD(+); NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); EC=2.4.2.30 |
Gene ID : | 142 |
mRNA Refseq : | NM_001618 |
Protein Refseq : | NP_001609 |
MIM : | 173870 |
UniProt ID : | P09874 |
Chromosome Location : | 1q41-q42 |
Pathway : | BER complex; BER complex; Base excision repair; Caspase cascade in apoptosis; FAS pathway and Stress induction of HSP regulation; Notch-mediated HES/HEY network |
Function : | DNA binding; NAD binding; NAD+ ADP-ribosyltransferase activity; metal ion binding; protein N-terminus binding; protein binding; transcription factor binding; transferase activity, transferring glycosyl groups; zinc ion binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionYes, overactivation of the PARP1 protein can lead to apoptosis or necrosis of cells, which may be exploited in the treatment of certain diseases.
The PARP1 protein can repair DNA damage by attaching ADP-ribose groups to damaged DNA strands and recruiting other repair proteins.
In addition to PARP1's involvement in direct DNA repair, it can also modulate the selection of repair pathways and initiate other DNA damage sensing mechanisms.
Some studies have proposed that the interaction of PARP1 protein with DNA damage in tumor immunotherapy may affect the efficacy of immunotherapy.
Yes, the PARP1 protein is involved in the normal regulation of the cell cycle, especially G1/S and G2/M switching during DNA damage.
Overactivation of PARP1 depletes energy stores such as NAD+ and ATP and leads to apoptosis, or cell death.
Customer Reviews (3)
Write a reviewThis range of applications of PARP1 is breathtaking, and it can play an important role in any field.
This short half-life and high clearance of PARP1 make it have greater potential in the treatment of tumors, infections and other diseases, and it is expected to become a new generation of therapeutic drugs.
The purity and bioactivity of PARP1 make it a valuable resource for scientific research.
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