Recombinant Human PPM1G, His-tagged

• Cat.No.: PPM1G-3564H
• Product Overview: Recombinant humanPPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: PPM1G-3564H
• Description: PPM1G,as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thrprotein phosphatases that is negative regulators of cell stress responsepathways. This enzyme localizes to the cytoplasm and is widely expressed,with most abundant expression detected in testis, skeletal muscle and heart.It is necessary for the dephosphorylation of pre-mRNA splicing factors, whichis an important process for the formation of the functional spliceosome.
• Form: Liquid. 20mM Tris-HClbuffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Weight: 61.4 kDa(566aa)
• Purity: > 85%by SDS - PAGE
• Concentration: 1.0mg/ml (determined by BCA assay)
• Biological activity: Specificactivity: >3,000 units/mg. Enzymatic activity was confirmed by measuringthe amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP)per minute at 37C, pH7.5 using 10mM of substrate.
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYDGHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKL TTEEV IKELAQIAGR PTEDEDEKEKVADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETPSQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEEEEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQLIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKNLPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELLDQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPM1G protein phosphatase,Mg2+/Mn2+ dependent, 1G [ Homo sapiens ]
• Official Symbol: PPM1G
• Synonyms: PPM1G; protein phosphatase, Mg2+/Mn2+dependent, 1G; PP2CG; PPP2CG; PP2CGAMMA; protein phosphatase 1G; PP2C-gamma;PP2C, gamma; MGC1675; protein phosphatase 1C; protein phosphatase 2C gammaisoform; protein phosphatase 2C isoform gamma; protein phosphatasemagnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gammaisoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gammaisoform; EC 3.1.3.16; OTTHUMP00000123426
• Gene ID: 5496
• mRNA Refseq: NM_177983
• Protein Refseq: NP_817092
• MIM: 605119
• UniProt ID: O15355
• Chromosome Location: 2p23.3
• Pathway: mRNA processing
• Function: catalytic activity; hydrolase activity;metal ion binding; protein serine/threonine phosphatase activity

Reviews

11/14/2022

PPM1G protein's utility in protein electron microscopy structure analysis is commendable.

12/03/2019

It enables researchers to delve deeper into the intricacies of protein behavior and aids in advancing our knowledge in various fields, such as molecular biology, biochemistry, and structural biology.

11/11/2019

It demonstrates excellent performance in ELISA assays, making it an ideal choice for researchers studying protein-protein interactions, antibody detection, or biomarker identification.

Q&As

Can PPM1G be targeted for cancer treatment?

Research is ongoing to explore the therapeutic potential of targeting PPM1G in cancer. Inhibiting or modulating its activity may be a strategy for cancer therapy.

Are there any known associations between PPM1G and cancer?

Yes, abnormal expression of PPM1G has been linked to certain cancers, suggesting its potential role as a biomarker or therapeutic target.

Are there any genetic disorders associated with PPM1G mutations?

While rare, mutations in the PPM1G gene have been reported and may be associated with certain genetic disorders. Further research is needed to understand the implications.

How does PPM1G contribute to DNA repair mechanisms?

PPM1G is involved in the regulation of DNA repair processes, influencing the repair of damaged DNA and maintaining genomic stability.

Can PPM1G be used as a diagnostic marker for specific diseases?

PPM1G expression levels may serve as a diagnostic marker for certain diseases, including some cancers, but more studies are required to establish its diagnostic utility.