Recombinant Human PPM1G, His-tagged

• Cat.No.: PPM1G-46H
• Product Overview: Recombinant Human protein phosphatase 1G fused with His-tag at N-terminus produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 27 kDa. The PRMT1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PPM1G-46H
• Description: PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.
• Source: Escherichia Coli.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD.
• Physical Appearance: Sterile Filtered colorless solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The PPM1G solution (1 mg/ml) contains 25 mM Tris pH-7.5, 1 mM DTT, 1 mM EDTA, 2 mM β-ME and 20% glycerol.
• Stability: PPM1G although stable 14°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PPM1G protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform [ Homo sapiens ]
• Synonyms: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; PP2CG; PPP2CG; MGC1675; MGC2870; PP2CGAMMA; PPM1G; protein phosphatase 1G; PP2C, gamma; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; PP2Cgamma; PP2C-gamma; OTTHUMP00000123426; PPM1C; EC 3.1.3.16; Protein phosphatase 1C; Protein phosphatase 2C isoform gamma; Protein phosphatase magnesium-dependent 1 gamma
• Gene ID: 5496
• mRNA Refseq: NM_002707
• Protein Refseq: NP_002698
• MIM: 605119
• UniProt ID: O15355
• Function: protein serine/threonine phosphatase activity; manganese ion binding; magnesium ion binding; hydrolase activity

Reviews

11/14/2022

PPM1G protein's utility in protein electron microscopy structure analysis is commendable.

12/03/2019

It enables researchers to delve deeper into the intricacies of protein behavior and aids in advancing our knowledge in various fields, such as molecular biology, biochemistry, and structural biology.

11/11/2019

It demonstrates excellent performance in ELISA assays, making it an ideal choice for researchers studying protein-protein interactions, antibody detection, or biomarker identification.

Q&As

Can PPM1G be targeted for cancer treatment?

Research is ongoing to explore the therapeutic potential of targeting PPM1G in cancer. Inhibiting or modulating its activity may be a strategy for cancer therapy.

Are there any known associations between PPM1G and cancer?

Yes, abnormal expression of PPM1G has been linked to certain cancers, suggesting its potential role as a biomarker or therapeutic target.

Are there any genetic disorders associated with PPM1G mutations?

While rare, mutations in the PPM1G gene have been reported and may be associated with certain genetic disorders. Further research is needed to understand the implications.

How does PPM1G contribute to DNA repair mechanisms?

PPM1G is involved in the regulation of DNA repair processes, influencing the repair of damaged DNA and maintaining genomic stability.

Can PPM1G be used as a diagnostic marker for specific diseases?

PPM1G expression levels may serve as a diagnostic marker for certain diseases, including some cancers, but more studies are required to establish its diagnostic utility.