Recombinant Human Protein Tyrosine Phosphatase, Receptor Type, F

• Cat.No.: PTPRF-1911H
• Product Overview: Recombinant humanPTPRF is expressed in E. coli; it contains the catalytic domain of PTPRF(residues 1330-1620) and has a molecular weight of 32 kDa.

Specification

• Cat. No.: PTPRF-1911H
• Description: PTPRF (leukocyteantigen related protein tyrosine phophatase) belongs to the protein-tyrosinephosphatase family, receptor class 2A subfamily. It possesses an intrinsicprotein tyrosine phosphatase activity (PTPase). The first PTPase domain hasenzymatic activity, while the second one seems to affect the substratespecificity of the first one. It is a possible cell adhesion receptor; itinteracts with GRIP1 (by similarity), PPFIA1, PPFIA2 and PPFIA3. Human PTPRFis a receptor protein tyrosine phosphatase containing 1897 amino acids.
• Source: E. coli
• Species: Human
• Form: 25 mM Tris-HCl (pH8.0), 75 mM NaCl, 0.05% Tween-20, 2 mM EDTA, 1 mM DTT and 50% glycerol.Liquid at -20°C.
• Purity: Greater than 95% asdetermined by Coomassie-stained SDS-PAGE gel.
• Specific Activity: 1.2 U/μg. One unitwill hydrolyze 1 nmol of p-nitrophenyl phosphate per minute at pH 7.4 and30°C. Assay buffer: 50 mM HEPES (pH 7.4), 2 mM EDTA, 3 mM DTT, 100 mM NaCland 50 mM pNPP.
• Applications: Removal ofphosphates from target substrates. Useful for the study of enzyme kinetics,regulation and for inhibitors screening.
• Storage: 6 months at -20°C.
• Pathways: Adherens junction;Cell adhesion molecules (CAMs); Insulin Signaling; Insulin signaling pathway

Gene Information

• Gene Name: PTPRFprotein tyrosine phosphatase, receptor type, F [ Homo sapiens ]
• Official Symbol: PTPRF
• Synonyms: PTPRF; proteintyrosine phosphatase, receptor type, F; LAR; receptor-typetyrosine-protein phosphatase F; LCA-homolog; OTTHUMP00000008683; OTTHUMP00000008684;OTTHUMP00000008688; OTTHUMP00000192913; OTTHUMP00000192914; leukocyte commonantigen related; leukocyte antigen-related (LAR) PTP receptor; leukocyteantigen-related tyrosine phosphatase; receptor-linked protein-tyrosinephosphatase LAR; protein tyrosine phosphatase, receptor type, F polypeptide
• Gene ID: 5792
• mRNA Refseq: NM_002840
• Protein Refseq: NP_002831
• MIM: 179590
• UniProt ID: P10586
• Chromosome Location: 1p34
• Function: hydrolase activity;insulin receptor binding; phosphate ion binding; protein tyrosine phosphataseactivity; receptor activity; receptor tyrosine kinase binding; transmembranereceptor protein tyrosine phosphatase activity

Reviews

06/11/2022

Qualitative is very strong, even after a lot of use can still maintain its structural and functional stability, which shows its excellent quality and reliability.

04/13/2022

The reproducibility is very good, and I can get consistent results from each experiment, which makes me more confident in the experimental results.

02/19/2022

Excellent performance in Western blot, avoiding interference problems in the experiment.

Q&As

Do specific PTPRF inhibitors have therapeutic effects on autoimmune diseases?

PTPRF inhibitors can inhibit certain abnormal signaling pathways in autoimmune diseases, thereby exerting their role in the treatment of autoimmune diseases.

What is the therapeutic effect of specific PTPRF inhibitors on inflammatory diseases?

Specific PTPRF inhibitors can inhibit certain signaling pathways in the inflammatory response, thereby playing a role in the treatment of inflammatory diseases.

What is the effect of knocking out the PTPRF gene on tumorigenesis?

Knocking out the PTPRF gene may increase the risk of developing certain tumors, which may be related to aberrant regulation of cell cycle control and cell growth.

Is there a specific PTPRF inhibitor?

There are currently some studies that report specific PTPRF inhibitors that can inhibit the enzymatic activity of PTPRF and thus exert therapeutic effects.

Is there a soluble PTPRF protein?

Yes, soluble PTPRF proteins exist that can be detected in extracellular fluids and may play a role in regulating cell-to-cell communication and signal transduction.

What are the therapeutic strategies for PTPRF protein?

Therapeutic strategies for PTPRF protein include increasing its expression level, inhibiting its enzymatic activity, and regulating its interaction with other proteins, which can be used to treat PTPRF protein-related diseases.