Recombinant Human RAB10, His-tagged

• Cat.No.: RAB10-7263H

Specification

• product overview: Recombinant human RAB10 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• description: RAB10 is a member of the Ras superfamily of small GTP-binding proteins. This protein is a key regulator of endocytic recycling, especially in the basolateral recycling pathways of polarized epithelial cells. It is required for the recruitment of CNT-1 to endosomal membranes in the intestinal epithelium.
• source: E. coli
• species: Human
• tag: His
• form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea
• molecular mass: 24.7 kDa(220aa)
• protein length: 1-200aa
• aa sequence: MGSSHHHHHH SSGLVPRGSH MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQERFHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIEKAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
• purity: >90% by SDS - PAGE
• applications: SDS-PAGE
• storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• concentration: 0.5 mg/ml

Gene Information

• gene name: RAB10 RAB10, member RAS oncogene family [ Homo sapiens ]
• official symbol: RAB10
• synonyms: RAB10; RAB10, member RAS oncogene family; ras-related protein Rab-10; ras related GTP binding protein; GTP-binding protein RAB10; ras-related GTP-binding protein
• Gene ID: 10890
• mrna refseq: NM_016131
• protein refseq: NP_057215
• mim: 612672
• uniprot id: P61026
• chromosome location: 2p23.3
• function: GTP binding; nucleotide binding

Reviews

04/12/2021

Reliable mass spectrometry analysis, pivotal for advancing our proteomics research.

07/07/2019

Accurate protein structural analysis, indispensable for our investigations.

10/17/2018

Swift and reliable protein sequencing, a key asset for our work.

Q&As

Did you check the phosphorylation status of the produced protein?

We didn't check the phosphorylation status of our RAB10 proteins. But the following one expressed in HEK293 should be phosphorylated.

How does RAB10 contribute to the process of autophagy and cellular homeostasis?

RAB10 aids in autophagy, maintaining cellular health by regulating the degradation and recycling of cell components.

What role does RAB10 play in insulin-stimulated glucose uptake and metabolic regulation?

RAB10 plays a crucial role in glucose uptake and metabolism, particularly in response to insulin.

What are the mechanisms by which RAB10 modulates cell signaling and communication?

RAB10 regulates cell signaling pathways, playing a role in intercellular communication and response to stimuli.

How does RAB10 influence the development and progression of neurodegenerative diseases?

RAB10 is linked to neurodegenerative diseases, potentially influencing protein aggregation and neuronal function.

How do alterations in RAB10 expression or activity affect the pathogenesis of diabetes and insulin resistance?

Variations in RAB10 can contribute to the development of diabetes and insulin resistance by affecting glucose metabolism.

How does RAB10 (Ras-Related Protein Rab-10) facilitate intracellular vesicle trafficking and membrane transport?

RAB10 is key in directing vesicle trafficking, ensuring efficient transport and membrane dynamics within cells.

What is the impact of RAB10 on endocytic pathways and receptor recycling?

RAB10 impacts endocytosis and receptor trafficking, affecting cell surface receptor dynamics and signaling.