Recombinant Human RAF1 Protein, N-terminal 6XHis and Avi Tag

• Cat.No.: RAF1-17H
• Product Overview: Ras Binding Domain (RBD) of Human Raf-1 Proto-Oncogene, Serine/Threonine Kinase (RAF1), (UniProt # P04049, amino acids 50-140) with the N-terminal Avi Tag peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme.

Specification

• Description: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
• Source: E. coli
• Species: Human
• Tag: The N-terminal 6XHis and Avi Tag
• Molecular Mass: ~13.4 kDa
• Protein Length: 50-140
• Purity: > 90% by Coomassie staining
• Applications: Measurement of the GTP/GDP bound ratio of Ras in vitro.
  Quantitation of GTP-bound Ras from tissue and tissue culture cell lysates.
  RAS/RAF binding studies.
  Inhibitor screening for RAS/RAF interaction
• Notes: This product is for laboratory research use only.
• Storage: Storage is recommended at -80 centigrade for longer periods of time.
• Storage Buffer: 20 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM 2-mercaptoethanol, 1 mM MgCl2, and 10% glycerol
  Purity: >90% by Coomassie staining
• Shipping: Product requires shipping on ice packs.

Gene Information

• Gene Name: RAF1 Raf-1 proto-oncogene, serine/threonine kinase [ Homo sapiens (human) ]
• Official Symbol: RAF1
• Synonyms: RAF1; Raf-1 proto-oncogene, serine/threonine kinase; NS5; CRAF; Raf-1; c-Raf; CMD1NN; RAF proto-oncogene serine/threonine-protein kinase; C-Raf proto-oncogene, serine/threonine kinase; Oncogene RAF1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1; EC 2.7.11.1
• Gene ID: 5894
• mRNA Refseq: NM_002880
• Protein Refseq: NP_002871
• MIM: 164760
• UniProt ID: P04049