Recombinant Human RASA1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RASA1-3433H
• Product Overview: RASA1 MS Standard C13 and N15-labeled recombinant protein (NP_002881) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 116.4 kDa
• AA Sequence: MMAAEAGSEEGGPVTAGAGGGGAAA GSSAYPAVCRVKIPAALPVAAAPYP GLVETGVAGTLGGGAALGSEFLGAG SVAGALGGAGLTGGGTAAGVAGAAA GVAGAAVAGPSGDMALTKLPTSLLA ETLGPGGGFPPLPPPPYLPPLGAGL GTVDEGDSLDGPEYEEEEVAIPLTA PPTNQWYHGKLDRTIAEERLRQAGK SGSYLIRESDRRPGSFVLSFLSQMN VVNHFRIIAMCGDYYIGGRRFSSLS DLIGYYSHVSCLLKGEKLLYPVAPP EPVEDRRRVRAILPYTKVPDTDEIS FLKGDMFIVHNELEDGWMWVTNLRT DEQGLIVEDLVEEVGREEDPHEGKI WFHGKISKQEAYNLLMTVGQVCSFL VRPSDNTPGDYSLYFRTNENIQRFK ICPTPNNQFMMGGRYYNSIGDIIDH YRKEQIVEGYYLKEPVPMQDQEQVL NDTVDGKEIYNTIRRKTKDAFYKNI VKKGYLLKKGKGKRWKNLYFILEGS DAQLIYFESEKRATKPKGLIDLSVC SVYVVHDSLFGRPNCFQIVVQHFSE EHYIFYFAGETPEQAEDWMKGLQAF CNLRKSSPGTSNKRLRQVSSLVLHI EEAHKLPVKHFTNPYCNIYLNSVQV AKTHAREGQNPVWSEEFVFDDLPPD INRFEITLSNKTKKSKDPDILFMRC QLSRLQKGHATDEWFLLSSHIPLKG IEPGSLRVRARYSMEKIMPEEEYSE FKELILQKELHVVYALSHVCGQDRT LLASILLRIFLHEKLESLLLCTLND REISMEDEATTLFRATTLASTLMEQ YMKATATQFVHHALKDSILKIMESK QSCELSPSKLEKNEDVNTNLTHLLN ILSELVEKIFMASEILPPTLRYIYG CLQKSVQHKWPTNTTMRTRVVSGFV FLRLICPAILNPRMFNIISDSPSPI AARTLILVAKSVQNLANLVEFGAKE PYMEGVNPFIKSNKHRMIMFLDELG NVPELPDTTEHSRTDLSRDLAALHE ICVAHSDELRTLSNERGAQQHVLKK LLAITELLQQKQNQYTKTNDVRSGP TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RASA1 RAS p21 protein activator 1 [ Homo sapiens (human) ]
• Official Symbol: RASA1
• Synonyms: RASA1; RAS p21 protein activator (GTPase activating protein) 1; RASA; ras GTPase-activating protein 1; capillary malformation arteriovenous malformation; CM AVM; GAP; p120GAP; p120RASGAP; triphosphatase-activating protein; PKWS; CMAVM; CM-AVM; RASGAP; DKFZp434N071
• Gene ID: 5921
• mRNA Refseq: NM_002890
• Protein Refseq: NP_002881
• MIM: 139150
• UniProt ID: P20936

Reviews

10/28/2019

The manufacturer's reliable quality allows me to conduct experiments with confidence.

09/01/2018

This protein reagent exhibits impeccable performance, providing stable and accurate results for every experiment.

07/09/2018

I am grateful for the outstanding performance and stable quality of this protein substance, which has greatly facilitated my research work.