Recombinant Human RBP3 protein, His-tagged

• Cat.No.: RBP3-3912H
• Product Overview: Recombinant Human RBP3 protein(898-1247 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 898-1247 aa
• AA Sequence: AMSATTGKAWDLAGVEPDITVPMSE ALSIAQDIVALRAKVPTVLQTAGKL VADNYASAELGAKMATKLSGLQSRY SRVTSEVALAEILGADLQMLSGDPH LKAAHIPENAKDRIPGIVPMQIPSP EVFEELIKFSFHTNVLEDNIGYLRF DMFGDGELLTQVSRLLVEHIWKKIM HTDAMIIDMRFNIGGPTSSIPILCS YFFDEGPPVLLDKIYSRPDDSVSEL WTHAQVVGERYGSKKSMVILTSSVT AGTAEEFTYIMKRLGRALVIGEVTS GGCQPPQTYHVDDTNLYLTIPTARS VGASDGSSWEGVGVTPHVVVPAEEA LARAKEMLQHNQLRVKRSPGLQDHL
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: RBP3 retinol binding protein 3, interstitial [ Homo sapiens ]
• Official Symbol: RBP3
• Synonyms: RBP3; retinol binding protein 3, interstitial; retinol-binding protein 3; D10S64; D10S65; D10S66; interphotoreceptor retinoid-binding protein; IRBP; RBPI
• Gene ID: 5949
• mRNA Refseq: NM_002900
• Protein Refseq: NP_002891
• MIM: 180290
• UniProt ID: P10745

Reviews

06/15/2022

Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

12/08/2018

Replicating results underscored the reliability and relevance of the product's effects.

09/07/2018

The user-friendly design of the product made it accessible to researchers with varying levels of expertise.

Q&As

How does RBP3 contribute to the efficient transport and delivery of retinoids to photoreceptor cells in the eye?

RBP3 contributes to the efficient transport and delivery of retinoids to photoreceptor cells, facilitating their uptake for visual processes.

Are there potential therapeutic strategies targeting RBP3 for vision-related disorders involving retinoid metabolism?

Potential therapeutic strategies targeting RBP3 could address vision-related disorders involving retinoid metabolism, making it a promising focus for drug development.

Are there known binding partners or receptors that interact with RBP3, influencing its role in retinoid transport and uptake?

RBP3 interacts with binding partners, including retinoids like 11-cis-retinal, influencing its role in retinoid transport and uptake.

In which ocular compartments is RBP3 predominantly localized, specifically within the retina?

RBP3 is predominantly localized within the retina, specifically in photoreceptor cells and the retinal pigment epithelium (RPE).

In what ways does RBP3 actively regulate retinoid homeostasis and participate in the visual cycle within the eye?

RBP3 actively regulates retinoid homeostasis and participates in the visual cycle, critical for maintaining optimal visual function.

What is the primary role of Retinol-Binding Protein 3 (RBP3) in supporting visual function and retinoid metabolism?

Retinol-Binding Protein 3 (RBP3) plays a crucial role in supporting visual function and regulating retinoid metabolism.

What vision-related disorders or conditions are associated with dysregulation or abnormal expression of RBP3?

Dysregulation or abnormal expression of RBP3 is associated with vision-related disorders such as retinitis pigmentosa.