Recombinant Human ROBO1 293 Cell Lysate

• Cat.No.: ROBO1-2256HCL

Specification

• Description: Antigen standard for roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: ROBO1 roundabout, axon guidance receptor, homolog 1 (Drosophila) [ Homo sapiens ]
• Official Symbol: ROBO1
• Synonyms: ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila); roundabout (axon guidance receptor, Drosophila) homolog 1; roundabout homolog 1; DUTT1; FLJ21882; SAX3; deleted in U twenty twenty; MGC131599; MGC133277
• Gene ID: 6091
• mRNA Refseq: NM_001145845
• Protein Refseq: NP_001139317
• MIM: 602430
• UniProt ID: Q9Y6N7
• Chromosome Location: 3p12.3
• Pathway: Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Inactivation of Cdc42 and Rac, organism-specific biosystem; Netrin-1 signaling, organism-specific biosystem
• Function: LRR domain binding; axon guidance receptor activity; identical protein binding; protein binding; protein heterodimerization activity; receptor activity

Reviews

06/28/2023

ROBO1 is able to maintain its biological activity and structural integrity under specific experimental conditions.

06/07/2022

ROBO1 products have low endotoxin levels and do not interfere with experimental results.

07/31/2021

When using ROBO1 for experiments, you can get a clear and reliable interpretation of your results.

Q&As

What is the association between ROBO1 recombinant protein and tumors?

Some studies have shown that ROBO1 recombinant protein may be involved in the initiation and progression of tumors, especially in neurological tumors.

Does ROBO1 recombinant protein function in pregnant women?

The level of ROBO1 recombinant protein in pregnant women may change due to the needs of fetal growth and development, and regular monitoring is required.

Does ROBO1 have a function in the human body?

ROBO1 is mainly involved in axon guidance and neural network formation in the human body, affecting the development and function of the nervous system.

If the level of ROBO1 recombinant protein is abnormal, what should I do?

If the level of ROBO1 recombinant protein is abnormal, treatment may include the use of drugs or other special treatments, depending on the condition and the doctor's recommendation.

Is there a treatment for ROBO1 recombinant proteins?

The current treatments for ROBO1 recombinant protein mainly include gene therapy, protein replacement therapy, and drug therapy for axon guidance.

What is the association between ROBO1 recombinant protein and neurological diseases?

ROBO1 recombinant protein may be involved in the occurrence and progression of neurological diseases, especially in glioma, neural tube defects, and autism.