Recombinant Human RTKN protein, His & GST-tagged

• Cat.No.: RTKN-8057H
• Product Overview: Recombinant Human RTKN aa. (Gln309~Phe416 (Accession # Q9BST9)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.2kDa
• Protein length: Gln309~Phe416 (Accession # Q9BST9)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 4.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: RTKN rhotekin [ Homo sapiens (human) ]
• Official Symbol: RTKN
• Synonyms: rhotekin
• Gene ID: 6242
• mRNA Refseq: NM_001015055.1
• Protein Refseq: NP_001015055.1
• UniProt ID: Q9BST9

Reviews

07/21/2022

They can offer specialized formulations, concentrations, or modifications of RTKN protein to suit unique experimental requirements.

03/23/2022

Collaborative discussions with the manufacturer can also help optimize experimental protocols and provide insights into the best practices in working with RTKN.

12/27/2021

The manufacturer can further support researchers by providing customized solutions tailored to their specific needs.

Q&As

Is RTKN a potential therapeutic target in cancer treatment?

Some studies propose RTKN as a potential therapeutic target for cancer, as inhibiting its activity may impede tumor progression.

How does RTKN contribute to cellular signaling pathways?

RTKN is known to modulate signaling pathways, including those associated with cell survival, proliferation, and differentiation.

How is RTKN linked to neurodegenerative diseases?

Some studies suggest that RTKN may be associated with neurodegenerative disorders, possibly influencing neuronal function.

Are there any known genetic mutations in the RTKN gene associated with diseases?

Genetic variations in the RTKN gene have been investigated for potential links to certain diseases, but more research is needed to establish clear associations.

Can RTKN be targeted for drug development?

The potential for developing drugs targeting RTKN is being explored, with a focus on its role in diseases such as cancer.