Recombinant Human SERPINA3 protein(Met1-Ala423), His-tagged

• Cat.No.: SERPINA3-665H
• Product Overview: Recombinant Human SERPINA3 (NP_001076.2) (Met 1-Ala 423) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ala423
• Form: Lyophilized from sterile 25mM HEPES, 0.15M NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is < 5 nM.
• Molecular Mass: The recombinant human SERPINA3 consisting of 409 amino acids and has a calculated molecular mass of 46.5 kDa. As a result of different glycosylation, the apparent molecular mass of the recombinant protein is approximately 45 kDa and 55-70 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SERPINA3 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 [ Homo sapiens ]
• Official Symbol: SERPINA3
• Synonyms: SERPINA3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; AACT, alpha 1 antichymotrypsin , serine (or cysteine) proteinase inhibitor, clade A (alpha 1 antiproteinase, antitrypsin), member 3; alpha-1-antichymotrypsin; ACT; alpha 1 antichymotrypsin; serpin A3; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3; AACT; GIG24; GIG25; MGC88254
• Gene ID: 12
• mRNA Refseq: NM_001085
• Protein Refseq: NP_001076
• MIM: 107280
• UniProt ID: P01011

Reviews

03/14/2022

The product's impact extended to data analysis, with intuitive tools for interpreting experimental results.

06/14/2020

The longevity of the product's effects allowed us to study cellular responses over extended timeframes.

10/31/2017

We found that the product's support for data analysis and visualization significantly expedited our research.

Q&As

What are the physiological roles of Serpina3n?

Serpina3n plays significant roles in various physiological processes, including modulation of the complement cascade, apoptosis, wound healing, and potential implications in Alzheimer's disease.

What is the significance of Serpina3n in pathologies?

Recent studies have reported significant roles of Serpina3n during various pathologies, particularly in central nervous system (CNS) insults, making it a potential therapeutic target.

What is the aim of literature drawing together Serpina3n applications?

The aim is to compile literature shedding light on the potential therapeutic applications of Serpina3n/SERPINA3 in various diseases. Additionally, a brief comparison between murine Serpina3n and its human ortholog SERPINA3 (α1-antichymotrypsin) is provided to account for their biological roles and significance.

Are the full range of Serpina3n applications known?

The full range of potential applications of Serpina3n is yet to be fully revealed, but its reported implications, especially in CNS insults, suggest potential therapeutic applications.

What is Serpina3n?

Serpina3n is a secretory serine protease inhibitor belonging to clade "a" with unique structural and physiological characteristics. |

How does Serpina3n inhibit proteases?

Serpina3n inhibits a wide range of proteases, contributing to its diverse physiological roles.

To which clade does Serpina3n belong?

Serpina3n belongs to clade "a" of serine protease inhibitors. |