Recombinant Human SOD3 293 Cell Lysate

• Cat.No.: SOD3-1573HCL

Specification

• Description: Antigen standard for superoxide dismutase 3, extracellular (SOD3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SOD3 superoxide dismutase 3, extracellular [ Homo sapiens ]
• Official Symbol: SOD3
• Synonyms: SOD3; superoxide dismutase 3, extracellular; extracellular superoxide dismutase [Cu-Zn]; EC SOD; EC-SOD; MGC20077
• Gene ID: 6649
• mRNA Refseq: NM_003102
• Protein Refseq: NP_003093
• MIM: 185490
• UniProt ID: P08294
• Chromosome Location: 4pter-q21
• Pathway: Folate Metabolism, organism-specific biosystem; Oxidative Stress, organism-specific biosystem; Selenium Pathway, organism-specific biosystem; superoxide radicals degradation, organism-specific biosystem
• Function: copper ion binding; heparin binding; metal ion binding; oxidoreductase activity; protein binding; superoxide dismutase activity; zinc ion binding

Reviews

02/19/2023

Trusted for results accuracy. A research necessity.

06/22/2017

Efficient and dependable. Accelerates our research progress.

06/12/2017

Enhances research credibility. Consistent and trustworthy.

Q&As

What potential therapeutic applications arise from targeting SOD3 in oxidative stress-related conditions?

Enhancing SOD3 activity could be a therapeutic strategy in treating oxidative stress-related diseases.

How do genetic variations in SOD3 affect its antioxidant function?

Genetic mutations in SOD3 can impair its ability to scavenge free radicals, affecting its protective role.

How does SOD3 contribute to cellular protection against free radicals?

It protects cells from oxidative damage by converting free radicals to less harmful substances.

What is the impact of altered SOD3 activity on aging and age-related diseases?

Altered SOD3 activity is linked with accelerated aging and the development of age-related diseases.

How does SOD3 interact with other cellular antioxidants?

SOD3 works alongside other antioxidants, contributing to the overall oxidative defense system.

What is the primary role of SOD3 in oxidative stress management?

SOD3, a type of superoxide dismutase, is crucial for neutralizing superoxide radicals, reducing oxidative stress.

What role does SOD3 play in cardiovascular health?

SOD3 is important for cardiovascular health, preventing oxidative damage in blood vessels.