Recombinant Human SPARC Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: SPARC-6051H
• Product Overview: SPARC MS Standard C13 and N15-labeled recombinant protein (NP_003109) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 34.6 kDa
• AA Sequence: MRAWIFFLLCLAGRALAAPQQEALP DETEVVEETVAEVTEVSVGANPVQV EVGEFDDGAEETEEEVVAENPCQNH HCKHGKVCELDENNTPMCVCQDPTS CPAPIGEFEKVCSNDNKTFDSSCHF FATKCTLEGTKKGHKLHLDYIGPCK YIPPCLDSELTEFPLRMRDWLKNVL VTLYERDEDNNLLTEKQKLRVKKIH ENEKRLEAGDHPVELLARDFEKNYN MYIFPVHWQFGQLDQHPIDGYLSHT ELAPLRAPLIPMEHCTTRFFETCDL DNDKYIALDEWAGCFGIKQKDIDKD LVITRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SPARC secreted protein acidic and cysteine rich [ Homo sapiens (human) ]
• Official Symbol: SPARC
• Synonyms: SPARC; secreted protein, acidic, cysteine-rich (osteonectin); ON; cysteine rich protein; osteonectin; BM-40; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• Gene ID: 6678
• mRNA Refseq: NM_003118
• Protein Refseq: NP_003109
• MIM: 182120
• UniProt ID: P09486