Recombinant Human TDG Protein, His-tagged

• Cat.No.: TDG-122H
• Product Overview: Recombinant Human TDG Protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 50mM Tris, 300mM NaCl, pH8.0.
• Molecular Mass: 26.5 kDa
• AA Sequence: MDDATNKKRKVFVSTILTFWNTDRR DFPWRHTRDPYVILITEILLRRTTA GHVKKIYDKFFVKYKCFEDILKTPK SEIAKDIKEIGLSNQRAEQLKELAR VVINDYGGRVPRNRKAILDLPGVGK YTCAAVMCLAFGKKAAMVDANFVRV INRYFGGSYENLNYNHKALWELAET LVPGGKCRDFNLGLMDFSAIICAPR KPKCEKCGMSKLCSYYEKCSTLEHH HHHH
• Purity: >90%
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.2 mg/ml

Gene Information

• Gene Name: TDG thymine DNA glycosylase [ Homo sapiens (human) ]
• Official Symbol: TDG
• Synonyms: hTDG
• Gene ID: 6996
• mRNA Refseq: NM_001363612
• Protein Refseq: NP_001350541
• MIM: 601423
• UniProt ID: Q13569