Recombinant Human TIAM1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TIAM1-634H
• Product Overview: TIAM1 MS Standard C13 and N15-labeled recombinant protein (NP_003244) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 177.3 kDa
• AA Sequence: MGNAESQHVEHEFYGEKHASLGRKH TSRSLRLSHKTRRTRHASSGKVIHR NSEVSTRSSSTPSIPQSLAENGLEP FSQDGTLEDFGSPIWVDRVDMGLRP VSYTDSSVTPSVDSSIVLTAASVQS MPDTEESRLYGDDATYLAEGGRRQH SYTSNGPTFMETASFKKKRSKSADI WREDSLEFSLSDLSQEHLTSNEEIL GSAEEKDCEEARGMETRASPRQLST CQRANSLGDLYAQKNSGVTANGGPG SKFAGYCRNLVSDIPNLANHKMPPA AAEETPPYSNYNTLPCRKSHCLSEG ATNPQISHSNSMQGRRAKTTQDVNA GEGSEFADSGIEGATTDTDLLSRRS NATNSSYSPTTGRAFVGSDSGSSST GDAARQGVYENFRRELEMSTTNSES LEEAGSAHSDEQSSGTLSSPGQSDI LLTAAQGTVRKAGALAVKNFLVHKK NKKVESATRRKWKHYWVSLKGCTLF FYESDGRSGIDHNSIPKHAVWVENS IVQAVPEHPKKDFVFCLSNSLGDAF LFQTTSQTELENWITAIHSACATAV ARHHHKEDTLRLLKSEIKKLEQKID MDEKMKKMGEMQLSSVTDSKKKKTI LDQIFVWEQNLEQFQMDLFRFRCYL ASLQGGELPNPKRLLAFASRPTKVA MGRLGIFSVSSFHALVAARTGETGV RRRTQAMSRSASKRRSRFSSLWGLD TTSKKKQGRPSINQVFGEGTEAVKK SLEGIFDDIVPDGKREKEVVLPNVH QHNPDCDIWVHEYFTPSWFCLPNNQ PALTVVRPGDTARDTLELICKTHQL DHSAHYLRLKFLIENKMQLYVPQPE EDIYELLYKEIEICPKVTQSIHIEK SDTAADTYGFSLSSVEEDGIRRLYV NSVKETGLASKKGLKAGDEILEINN RAADALNSSMLKDFLSQPSLGLLVR TYPELEEGVELLESPPHRVDGPADL GESPLAFLTSNPGHSLCSEQGSSAE TAPEETEGPDLESSDETDHSSKSTE QVAAFCRSLHEMNPSDQSPSPQDST GPQLATMRQLSDADKLRKVICELLE TERTYVKDLNCLMERYLKPLQKETF LTQDELDVLFGNLTEMVEFQVEFLK TLEDGVRLVPDLEKLEKVDQFKKVL FSLGGSFLYYADRFKLYSAFCASHT KVPKVLVKAKTDTAFKAFLDAQNPK QQHSSTLESYLIKPIQRILKYPLLL RELFALTDAESEEHYHLDVAIKTMN KVASHINEMQKIHEEFGAVFDQLIA EQTGEKKEVADLSMGDLLLHTTVIW LNPPASLGKWKKEPELAAFVFKTAV VLVYKDGSKQKKKLVGSHRLSIYED WDPFRFRHMIPTEALQVRALASADA EANAVCEIVHVKSESEGRPERVFHL CCSSPESRKDFLKAVHSILRDKHRR QLLKTESLPSSQQYVPFGGKRLCAL KGARPAMSRAVSAPSKSLGRRRRRL ARNRFTIDSDAVSASSPEKESQQPP GGGDTDRWVEEQFDLAQYEEQDDIK ETDILSDDDEFCESVKGASVDRDLQ ERLQATSISQRERGRKTLDSHASRM AQLKKQAALSGINGGLESASEEVIW VRREDFAPSRKLNTEISGPTRTRRL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TIAM1 T-cell lymphoma invasion and metastasis 1 [ Homo sapiens (human) ]
• Official Symbol: TIAM1
• Synonyms: TIAM1; T-cell lymphoma invasion and metastasis 1; T-lymphoma invasion and metastasis-inducing protein 1; TIAM-1; human T-lymphoma invasion and metastasis inducing TIAM1 protein; FLJ36302
• Gene ID: 7074
• mRNA Refseq: NM_003253
• Protein Refseq: NP_003244
• MIM: 600687
• UniProt ID: Q13009