Recombinant Human TLR4 Protein, His-tagged

• Cat.No.: TLR4-141H
• Product Overview: Recombinant Human TLR4 Protein with His tag was expressed in E. coli.

Specification

• Description: Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM) . This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Toll-like receptor 4 (TLR4) functions in association with MD-2 in the recognition and initiation of immune responses elicited by lipopolysaccharide (LPS) of Gram-negative bacteria. TLR4 triggers the activation of NF-κB, IRF-3, and MAPK pathways leading to the production of inflammatory cytokines.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~60 kDa
• AA Sequence: ESWEPCVEVVPNITYQCMELNFYKI PDNLPFSTKNLDLSFNPLRHLGSYS FFSFPELQVLDLSRCEIQTIEDGAY QSLSHLSTLILTGNPIQSLALGAFS GLSSLQKLVAVETNLASLENFPIGH LKTLKELNVAHNLIQSFKLPEYFSN LTNLEHLDLSSNKIQSIYCTDLRVL HQMPLLNLSLDLSLNPMNFIQPGAF KEIRLHKLTLRNNFDSLNVMKTCIQ GLAGLEVHRLVLGEFRNEGNLEKFD KSALEGLCNLTIEEFRLAYLDYYLD DIIDLFNCLTNVSSFSLVSVTIERV KDFSYNFGWQHLELVNCKFGQFPTL KLKSLKRLTFTSNKGGNAFSEVDLP SLEFLDLSRNGLSFKGCCSQSDFGT TSLKYLDLSFNGVITMSSNFLGLEQ LEHLDFQHSNLKQMSEFSVFLSLRN LIYLDISHTHTRVAFNGIFNGLSSL EVLKMAGNSFQENFLPDIFTELRNL TFLDLSQCQLEQLSPTAFNSLSSLQ VLNMSHNNFFSLDTFPYKCLNSLQV LDYSLNHIMTSKKQELQHFPSSLAF LNLTQNDFACTCEHQSFLQWIKDQR QLLVEVERMECATPSDKQGMPVLSL NITCQMNK
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: TLR4 toll-like receptor 4 [ Homo sapiens (human) ]
• Official Symbol: TLR4
• Synonyms: TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10
• Gene ID: 7099
• mRNA Refseq: NM_003266
• Protein Refseq: NP_003257
• MIM: 603030
• UniProt ID: O00206