Recombinant Human TOP1, His-tagged

• Cat.No.: TOP1-30912TH
• Product Overview: DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton (excluding glycosilation) 102 kDa total mass. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Specification

• Description: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
• Source: Sf9 insect cells.
• Species: Human
• Tag: His
• Form: Sterile Filtered clear solution. TOP1 is supplied in 16mM HEPES buffer pH-7.5, 400mM sodium chloride, and 20% glycerol.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
• Publication:
  Suppression of lupus nephritis and skin lesions in MRL/lpr mice by administration of the topoisomerase I inhibitor irinotecan (2016)
  The topoisomerase I inhibitor irinotecan and the tyrosyl‐DNA phosphodiesterase 1 inhibitor furamidine synergistically suppress murine lupus nephritis (2015)
  Low dose irinotecan improves advanced lupus nephritis in mice potentially by changing DNA relaxation and anti‐dsDNA binding. (2014)

Gene Information

• Gene Name: TOP1 topoisomerase (DNA) I [ Homo sapiens (human) ]
• Official Symbol: TOP1
• Synonyms: TOP1; TOPI; topoisomerase (DNA) I; DNA topoisomerase 1; type I DNA topoisomerase; NP_003277.1; EC 5.99.1.2
• Gene ID: 7150
• mRNA Refseq: NM_003286
• Protein Refseq: NP_003277
• MIM: 126420
• UniProt ID: P11387
• Chromosome Location: 20q12-q13.1
• Pathway: Caspase cascade in apoptosis; Integrated Pancreatic Cancer Pathway
• Function: ATP binding; DNA topoisomerase type I activity; DNA topoisomerase type II (ATP-hydrolyzing) activity