Recombinant Human TPX2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: TPX2-1921H
• Product Overview: TPX2 MS Standard C13 and N15-labeled recombinant protein (NP_036244) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 85.7 kDa
• AA Sequence: MSQVKSSYSYDAPSDFINFSSLDDE GDTQNIDSWFEEKANLENKLLGKNG TGGLFQGKTPLRKANLQQAIVTPLK PVDNTYYKEAEKENLVEQSIPSNAC SSLEVEAAISRKTPAQPQRRSLRLS AQKDLEQKEKHHVKMKAKRCATPVI IDEILPSKKMKVSNNKKKPEEEGSA HQDTAEKNASSPEKAKGRHTVPCMP PAKQKFLKSTEEQELEKSMKMQQEV VEMRKKNEEFKKLALAGIGQPVKKS VSQVTKSVDFHFRTDERIKQHPKNQ EEYKEVNFTSELRKHPSSPARVTKG CTIVKPFNLSQGKKRTFDETVSTYV PLAQQVEDFHKRTPNRYHLRSKKDD INLLPSKSSVTKICRDPQTPVLQTK HRARAVTCKSTAELEAEELEKLQQY KFKARELDPRILEGGPILPKKPPVK PPTEPIGFDLEIEKRIQERESKKKT EDEHFEFHSRPCPTKILEDVVGVPE KKVLPITVPKSPAFALKNRIRMPTK EDEEEDEPVVIKAQPVPHYGVPFKP QIPEARTVEICPFSFDSRDKERQLQ KEKKIKELQKGEVPKFKALPLPHFD TINLPEKKVKNVTQIEPFCLETDRR GALKAQTWKHQLEEELRQQKEAACF KARPNTVISQEPFVPKKEKKSVAEG LSGSLVQEPFQLATEKRAKERQELE KRMAEVEAQKAQQLEEARLQEEEQK KEELARLRRELVHKANPIRKYQGLE IKSSDQPLTVPVSPKFSTRFHCTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: TPX2 TPX2 microtubule nucleation factor [ Homo sapiens (human) ]
• Official Symbol: TPX2
• Synonyms: TPX2; TPX2, microtubule-associated, homolog (Xenopus laevis); C20orf1, C20orf2, chromosome 20 open reading frame 1; targeting protein for Xklp2; DIL 2; p100; protein fls353; differentially expressed in lung cells; TPX2, microtubule-associated protein homolog; preferentially expressed in colorectal cancer; hepatocellular carcinoma-associated antigen 519; restricted expression proliferation associated protein 100; restricted expression proliferation-associated protein 100; differentially expressed in cancerous and non-cancerous lung cells 2; DIL2; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
• Gene ID: 22974
• mRNA Refseq: NM_012112
• Protein Refseq: NP_036244
• MIM: 605917
• UniProt ID: Q9ULW0