Recombinant Human Tyrosine Kinase, Non-receptor, 2, GST-tagged, Active

• Cat.No.: ACK-234H
• Product Overview: Recombinant human ACK (110-476) was expressed by baculovirus inSf9 insect cellsusing an N-terminal GST tag. MW=66 kDa.

Specification

• Cat. No.: ACK-234H
• Description: ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs. Overexpression of ACK in cancer cell lines of epithelial origin increases cellular motility and invasiveness. In a mouse model, ACK overexpression enhances the ability of a human breast cancer cell line to metastasize to the lung and increased mortality. Ligand stimulation of alpha-3 beta-1 integrin leads to activation of ACK which then enhances p130CAS phosphorylation and activation of RAC.
• Source: Sf9 insect cells using baculovirus.
• Sequence: 110-476.
• Applications: Kinase Assay, Western Blot.
• Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: TNK2 tyrosine kinase, non-receptor, 2 [ Homo sapiens ]
• Synonyms: TNK2; tyrosine kinase, non-receptor, 2; ACK; ACK1; FLJ44758; FLJ45547; p21cdc42Hs; activated p21cdc42Hs kinase; activated Cdc42-associated kinase 1; EC 2.7.10.2
• Gene ID: 10188
• mRNA Refseq: NM_001010938
• Protein Refseq: NP_001010938
• MIM: 606994
• UniProt ID: Q07912
• Chromosome Location: 3q29
• Function: ATP binding; GTPase inhibitor activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; protein binding; transferase activity

Reviews

01/06/2023

Excellent experimental data was obtained using this product ACK!

01/23/2020

The product information is comprehensive and easy to use

Q&As

How does tyrosine kinase play a role in immune cell signaling and response?

Tyrosine kinase plays a crucial role in immune cell signaling and response. For example, activation of tyrosine kinase receptors on T cells and B cells can lead to downstream signaling pathways that regulate cell activation, proliferation, and differentiation. In addition, non-receptor tyrosine kinases such as Syk and ZAP-70 are critical for immune cell signaling and response.

What are the methods used to study tyrosine kinase activity?

There are several methods used to study tyrosine kinase activity, such as western blotting, immunoprecipitation, and kinase assays. These methods can detect changes in tyrosine kinase activity and downstream signaling pathways in response to different stimuli.

How do mutations in tyrosine kinase genes lead to cancer development?

Mutations in tyrosine kinase genes can lead to constitutive activation of tyrosine kinase, which can promote uncontrolled cell growth and tumorigenesis. This activation can occur through various mechanisms, such as ligand-independent activation, increased expression of the tyrosine kinase receptor, or loss of negative regulatory mechanisms.

What are the current drugs targeting tyrosine kinases?

There are several drugs that target tyrosine kinases, such as imatinib, erlotinib, and gefitinib. These drugs are used in the treatment of various cancers, such as chronic myeloid leukemia, non-small cell lung cancer, and gastrointestinal stromal tumors.

What are the diseases associated with aberrant tyrosine kinase activity?

Aberrant tyrosine kinase activity has been implicated in various diseases, including cancer, autoimmune disorders, and cardiovascular diseases. Mutations in tyrosine kinase genes can result in constitutive activation of tyrosine kinase, leading to uncontrolled cell growth and tumorigenesis.

What are the downstream effects of tyrosine kinase activation?

Activation of tyrosine kinase can lead to various downstream effects, such as changes in gene expression, cell proliferation, differentiation, and survival. These effects are mediated by downstream signaling pathways that are activated by phosphorylation of specific substrates.

How is the activity of tyrosine kinase regulated?

The activity of tyrosine kinase is regulated by various mechanisms, including ligand binding, dimerization, and phosphorylation. Inhibition of tyrosine kinase activity is also regulated by protein phosphatases that remove the phosphate group from the substrate protein.

What are the types of tyrosine kinase receptors?

There are two types of tyrosine kinase receptors: receptor tyrosine kinases (RTKs) and non-receptor tyrosine kinases. RTKs are transmembrane receptors that are activated by ligand binding, such as growth factors. Non-receptor tyrosine kinases are cytoplasmic proteins that are activated by intracellular signals, such as cytokines.

How does tyrosine kinase work in cell signaling pathways?

Tyrosine kinases are activated by ligand binding to their associated receptors on the cell surface. This activation leads to autophosphorylation of the tyrosine kinase domain, which then phosphorylates downstream substrates. The phosphorylated substrates recruit other signaling molecules to form signaling complexes that propagate the signal downstream to regulate cellular processes.