Recombinant Human WEE1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: WEE1-6052H
• Product Overview: WEE1 MS Standard C13 and N15-labeled recombinant protein (NP_003381) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 71.4 kDa
• AA Sequence: MSFLSRQQPPPPRRAGAACTLRQKL IFSPCSDCEEEEEEEEEEGSGHSTG EDSAFQEPDSPLPPARSPTEPGPER RRSPGPAPGSPGELEEDLLLPGACP GADEAGGGAEGDSWEEEGFGSSSPV KSPAAPYFLGSSFSPVRCGGPGDAS PRGCGARRAGEGRRSPRPDHPGTPP HKTFRKLRLFDTPHTPKSLLSKARG IDSSSVKLRGSSLFMDTEKSGKREF DVRQTPQVNINPFTPDSLLLHSSGQ CRRRKRTYWNDSCGEDMEASDYELE DETRPAKRITITESNMKSRYTTEFH ELEKIGSGEFGSVFKCVKRLDGCIY AIKRSKKPLAGSVDEQNALREVYAH AVLGQHSHVVRYFSAWAEDDHMLIQ NEYRNGGSLADAISENYRIMSYFKE AELKDLLLQVGRGLRYIHSMSLVHM DIKPSNIFISRTSIPNAASEEGDED DWASNKVMFKIGDLGHVTRISSPQV EEGDSRFLANEVLQENYTHLPKADI FALALTVVCAAGAEPLPRNGDQWHE IRQGRLPRIPQVLSQEFTELLKVMI HPDPERRPSAMALVKHSVLLSASRK SAEQLRIELNAEKFKNSLLQKELKK AQMAKAAAEERALFTDRMATRSTTQ SNRTSRLIGKKMNRSVSLTIYTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 homolog (S. pombe); wee1+ (S. pombe) homolog; wee1-like protein kinase; wee1A kinase; WEE1+ homolog; WEE1A; WEE1hu; FLJ16446; DKFZp686I18166
• Gene ID: 7465
• mRNA Refseq: NM_003390
• Protein Refseq: NP_003381
• MIM: 193525
• UniProt ID: P30291