| Description : |
Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks. Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
52.5 kDa |
| AA Sequence : |
MGRKKKKQLKPWCWYCNRDFDDEKI LIQHQKAKHFKCHICHKKLYTGPGL AIHCMQVHKETIDAVPNAIPGRTDI ELEIYGMEGIPEKDMDERRRLLEQK TQESQKKKQQDDSDEYDDDDSAAST SFQPQPVQPQQGYIPPMAQPGLPPV PGAPGMPPGIPPLMPGVPPLMPGMP PVMPGMPPGLHHQRKYTQSFCGENI MMPMGGMMPPGPGIPPLMPGMPPGM PPPVPRPGIPPMTQAQAVSAPGILN RPPAPTATVPAPQPPVTKPLFPSAG QMGTPVTSSSTASSNSESLSASSKA LFPSTAQAQAAVQGPVGTDFKPLNS TPATTTEPPKPTFPAYTQSTASTTS TTNSTAAKPAASITSKPATLTTTSA TSKLIHPDEDISLEERRAQLPKYQR NLPRPGQAPIGNPPVGPIGGMMPPQ PGIPQQQGMRPPMPPHGQYGGHHQG MPGYLPGAMPPYGQGPPMVPPYQGG PPRPPMGMRPPVMSQGGRYTRTRPL EQKLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
