Recombinant Monkey EGFR Protein, hFc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: EGFR-1226RAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant Rhesus macaque EGFR (Leu25-Ser645) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
• Source: HEK293
• Species: Monkey
• Tag: hFc
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 95.3 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Leu25-Ser645
• N-terminal Sequence Analysis: Leu 25
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR
• Official Symbol: EGFR
• Gene ID: 613027
• mRNA Refseq: XM_015133436
• Protein Refseq: XP_014988922
• UniProt ID: P55245