Recombinant Mouse Abcc1 protein, His & T7-tagged

• Cat.No.: Abcc1-1458M
• Product Overview: Recombinant Mouse Abcc1 aa. (Arg1219~Ile1469 (Accession # O35379)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein plays an essential role in the defense against toxic compounds and serves as the major high-affinity transporter of leukotriene C4. The encoded protein may also play an essential role in steroid hormone homeostasis as a transporter for steroid hormones and their metabolites.
• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.7kDa.
• Protein length: Arg1219~Ile1469 (Accession # O35379)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Abcc1 ATP-binding cassette, sub-family C (CFTR/MRP), member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Abcc1
• Synonyms: MRP; Mrp1; Mdrap; Abcc1a; Abcc1b; ATP-binding cassette, sub-family C (CFTR/MRP), member 1a; ATP-binding cassette, sub-family C (CFTR/MRP), member 1b; LTC4 transporter; leukotriene C(4) transporter; multiple drug resistance-associated protein; multidrug resistance-associated protein 1
• Gene ID: 17250
• mRNA Refseq: NM_008576.3
• Protein Refseq: NP_032602.1
• UniProt ID: O35379

Reviews

04/11/2022

The protein meets the custom requirements, great price and the technician can give me professional help.

10/09/2021

The technicians provided me with a lot of after-sales help, and the bands obtained for WB are also very clear.

12/18/2020

Just participated in this promotion. High protein purity and good experimental results.

11/07/2020

Right price, fast delivery and good after-sales service.

07/05/2019

Fast delivery and high quality product.

Q&As

Can I customize ABCC1 protein with other tags?

Yes, you can communicate with our experts about specific customization needs.

When will it arrive after my purchase?

In-stock products will be shipped as soon as possible after your purchase, and the specific arrival time will vary from address to address.

How should the protein products of lyophilized powder be handled? Will there be relevant information in the product manual?

Our product manuals generally include relevant information, or you can contact our technical experts at any time and they will be able to give you relevant assistance.

Can I choose to express specific fragments of the ABCC1 protein?

You can communicate directly with our experts.

Is ABCC1 protein participating in this Thanksgiving promotion? What about ABCC1 protein that requires custom production?

Yes, ABCC1 protein, including custom-produced ABCC1 protein, is participating in this promotion.

How should I choose between ABCC1 protein products with different parameters?

Our experts can advise on your research needs.

How are the proteins you provide purified?

Protein purification is generally performed by hydrophobic chromatography, affinity chromatography, ion exchange chromatography, salting out, molecular sieve, etc.

What protein expression systems do you offer?

We offer a wide range of protein expression systems, including common bacterial expression systems, baculovirus-insect cell expression systems, cell-free in vitro protein expression systems, mammalian expression systems, yeast expression systems, etc.

In which tissues is the ABCC1 protein expressed?

ABCC1 protein is ubiquitously expressed in most normal tissues.

I need to buy more than one protein, is there a discount?

You can communicate directly with us about your specific purchase needs, and we will determine if there is a discount based on the actual situation.

Do you offer other MRP family proteins?

We also offer ABCC2, ABCC3, ABCC4, ABCC6 proteins, or you can get the protein you need through our custom protein production service.

What can I use to block before primary antibody incubation in WB?

Membrane can be blocked in 5% milk before incubation with primary antibody.

What kind of membrane is generally used for WB with ABCC1?

Generally, nitrocellulose membrane and PVDF membrane are used for Western Blot.

ABCC1 protein is commonly used in multidrug resistance studies. Is there a correlation between multidrug resistance and ABCC1 protein expression levels?

Several studies have shown that multidrug resistance is associated with enhanced ABCC1 protein expression.

Does ABCC1 cause resistance to broad-spectrum compounds?

Yes, ABCC1 causes resistance to natural product drugs such as epipodophyllotoxins, vinca alkaloids and certain anthracyclines.

What other proteins are in the MRP family?

ABCC1-6, ABCC10-13 all belong to the MRP family.