Recombinant Mouse ABCG8 Protein Pre-coupled Magnetic Beads

• Cat.No.: ABCG8-209M-B
• Product Overview: The Recombnant protein was conjugated to magnetic beads. This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. This product can be equipped with automation equipment for high-throughput operations.

Specification

• Source: HEK293
• Species: Mouse
• Form: Solution
• Particle size: ~2 μm
• Beads Surface: Hydrophilic
• Capacity: > 200 pmol rabbit IgG/ mg beads
• Applications: Immunoassay, In vitro diagnostics, cell sorting, Immunoprecipitation/Co-precipitation, Protein/antibody separation and purification.
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions.
• Storage: 2-8℃. Do not to freeze thaw the Beads
• Concentration: 10mg beads/mL
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Abcg8 ATP-binding cassette, sub-family G (WHITE), member 8 [ Mus musculus ]
• Official Symbol: ABCG8
• Gene ID: 67470
• mRNA Refseq: NM_026180.2
• Protein Refseq: NP_080456.1
• UniProt ID: Q9DBM0

Reviews

08/01/2019

Good work in immunohistochemistry

04/21/2018

The purification rsults demonstrtaed clearly! Great.

Q&As

What may happen if the allele in ABCG8, NPC1L1 rs217434 mutated?

To a lesser extent, NPC1L1 rs217434 variants increase the risk of early-onset gallstone formation.

what biological variation may influence the association between ABCG8 transporters and hypercholesterolemia?

Single nucleotide polymorphisms (SNPs) in ABCG8 transporter have been associated with hypercholesterolemia.

What will happen if disrupt both ABCG5 and ABCG8 in mice?

lt will cause extremely low biliary cholesterol concentrations while biliary phospholipids and bile acid concentrations are not altered.

What is the relationship between ABCG8 and leptin in mice?

Mice lacking leptin, exhibit decreased abundance of ABCG8 proteins in the liver.

What will the overexpression of ABCG8 genes cause?

It will potentially induce atherosclerosis because of reduced circulating and hepatic cholesterol.

What results can you found through western blot experiment results?

both anti-ABCG5 and anti-ABCG8 antibodies bound to ~75 kDa proteins in human liver crude membranes.

What is the difference between deficient in either Abcg5 or Abcg8 and double missing?

One study constructed mouse models deficient in either Abcg5 or Abcg8 that show different biliary physiology than that of the Abcg5/Abcg8 double knockout mice. This suggests these proteins may have independent function(s) in addition to their function as heterodimers.

What is the distribution pattern of ABCG8 in small intestine ?

 ABCG8 appear to line the brush border.

What changes can affect the expression of ABCG8?

Disruptions of bile acid metabolism alter hepatic ABCG5/G8 expression.

What is the relationship between ABCG5 and ABCG8?

ABCG5 and ABCG8 are co-regulated at the transcriptional level, sharing a common bidirectional promoter of 374 base pairs that separates their initiation codons, cause sitosterolemia as well.