Recombinant Mouse Acaa1b Protein, Myc/DDK-tagged

• Cat.No.: Acaa1b-1483M
• Product Overview: Purified recombinant protein of mouse full-length acetyl-Coenzyme A acyltransferase 1B (Acaa1b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Biased expression in liver adult (RPKM 383.0), kidney adult (RPKM 114.5) and 8 other tissues
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 44 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Acaa1b acetyl-Coenzyme A acyltransferase 1B [ Mus musculus (house mouse) ]
• Official Symbol: Acaa1b
• Synonyms: Acaa1b; acetyl-Coenzyme A acyltransferase 1B; 3-ketoacyl-CoA thiolase B, peroxisomal; acetyl-CoA acyltransferase B; beta-ketothiolase B; peroxisomal 3-oxoacyl-CoA thiolase B; EC 2.3.1.155; EC 2.3.1.16; EC 2.3.1.9
• Gene ID: 235674
• mRNA Refseq: NM_146230
• Protein Refseq: NP_666342
• UniProt ID: Q8VCH0

Reviews

03/30/2022

Shows good stability and compatibility with various sample types and buffers.

09/05/2021

Demonstrates excellent specificity and sensitivity in detecting the target protein in sandwich ELISAs.

05/02/2016

Highly efficient in neutralizing the activity of the target protein in bioassays.

Q&As

What is the structure of the acetyl-Coenzyme A acyltransferase 1B protein?

The acetyl-Coenzyme A acyltransferase 1B protein structure is composed of an N-terminal domain, a central domain, and a C-terminal domain.

What is the tissue distribution of acetyl-Coenzyme A acyltransferase 1B expression?

Acetyl-Coenzyme A acyltransferase 1B is mainly expressed in liver and adipose tissue, but also in other tissues such as muscle and brain.

How is acetyl-Coenzyme A acyltransferase 1B involved in insulin sensitivity and diabetes?

Acetyl-Coenzyme A acyltransferase 1B is involved in regulating insulin sensitivity and glucose homeostasis, and dysregulation of this protein has been implicated in the development of diabetes.

How is acetyl-Coenzyme A acyltransferase 1B regulated?

Acetyl-Coenzyme A acyltransferase 1B is regulated by post-translational modifications, transcriptional regulation, and by various signaling pathways.

What is the role of acetyl-Coenzyme A acyltransferase 1B in lipid metabolism?

Acetyl-Coenzyme A acyltransferase 1B plays a key role in lipid metabolism by catalyzing the conversion of acetyl-CoA and fatty acid CoA thioesters to produce long-chain acyl-CoAs, which are then used for energy production or storage.

What are the substrates of acetyl-Coenzyme A acyltransferase 1B?

The substrates of acetyl-Coenzyme A acyltransferase 1B are acetyl-CoA and fatty acid CoA thioesters.

What diseases are associated with mutations in the acetyl-Coenzyme A acyltransferase 1B gene?

Mutations in the acetyl-Coenzyme A acyltransferase 1B gene have been associated with metabolic disorders such as obesity and insulin resistance.

What is the relationship between acetyl-Coenzyme A acyltransferase 1B and fatty acid synthesis?

Acetyl-Coenzyme A acyltransferase 1B is not directly involved in fatty acid synthesis, but it plays a role in the regulation of fatty acid metabolism and the synthesis of complex lipids.

What is the function of acetyl-Coenzyme A acyltransferase 1B protein?

The function of acetyl-Coenzyme A acyltransferase 1B protein is to catalyze the conversion of acetyl-CoA and fatty acid CoA thioesters to produce long-chain acyl-CoAs.

What is the evolutionary history of the acetyl-Coenzyme A acyltransferase 1B gene and protein?

The acetyl-Coenzyme A acyltransferase 1B gene and protein are conserved throughout evolution and are found in a wide range of organisms, from bacteria to humans.